| 纯度 | >80%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HSPBAP1 |
| Uniprot No | Q96EW2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1- 488aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMAAGSEATTPVIVAAGAGGEEGEHVKPFKP EKAKEIIMSLQQPAIFCNMVFDWPARHWNAKYLSQVLHGKQIRFRMGMKS MSTVPQFETTCNYVEATLEEFLTWNCDQSSISGPFRDYDHSKFWAYADYK YFVSLFEDKTDLFQDVKWSDFGFPGRNGQESTLWIGSLGAHTPCHLDSYG CNLVFQVQGRKRWHLFPPEDTPFLYPTRIPYEESSVFSKINVVNPDLKRF PQFRKAQRHAVTLSPGQVLFVPRHWWHYVESIDPVTVSINSWIELEEDHL ARVEEAITRMLVCALKTAENPQNTRAWLNPTEVEETSHAVNCCYLNAAVS AFFDRCRTSEVVEIQALRTDGEHMKKEELNVCNHMEVGQTGSQNLTTGTD KPEAASPFGPDLVPVAQRSEEPPSERGGIFGSDGKDFVDKDGEHFGKLHC AKRQQIMSNSENAIEEQIASNTTTTPQTFISTDDLLDCLVNPQVTRIVAQ LLIQGRSL |
| 预测分子量 | 57 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HSPBAP1重组蛋白的3篇示例参考文献(注：以下为虚构示例，实际文献请通过学术数据库查询)：
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1. **标题**: *"Expression and functional characterization of recombinant HSPBAP1 in cellular stress response"*
**作者**: Zhang Y. et al.
**摘要**: 本研究通过原核表达系统成功纯化HSPBAP1重组蛋白，发现其与HSPB1相互作用并增强细胞在热休克条件下的存活率，提示其在氧化应激中的分子伴侣功能。
2. **标题**: *"HSPBAP1 promotes tumor metastasis via modulating JNK signaling pathway"*
**作者**: Li X. et al.
**摘要**: 利用重组HSPBAP1蛋白过表达模型，证实其通过激活JNK通路促进癌细胞迁移和侵袭，为癌症治疗提供了潜在靶点。
3. **标题**: *"Structural analysis of HSPBAP1 reveals a novel phosphorylation-dependent regulatory mechanism"*
**作者**: Chen L. et al.
**摘要**: 通过晶体学解析HSPBAP1重组蛋白结构，发现其C端结构域的磷酸化修饰影响与HSP27的结合能力，揭示了其在神经退行性疾病中的潜在作用机制。
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建议通过PubMed、Web of Science或Google Scholar检索关键词 "HSPBAP1 recombinant protein" 或 "HSPBAP1 functional study" 获取真实文献。
HSPBAP1 (HSPB1-associated protein 1), also known as HSPBP1. is a protein that interacts with the small heat shock protein HSPB1 (HSP27). It plays a role in modulating the chaperone activity and cellular functions of HSPB1. which is involved in stress response, apoptosis, and cytoskeletal organization. HSPBAP1 contains a conserved ubiquitin-associated (UBA) domain, suggesting its potential involvement in protein quality control pathways, including the ubiquitin-proteasome system. Studies indicate that HSPBAP1 acts as a co-chaperone, regulating HSPB1’s ability to stabilize client proteins under stress conditions, though its precise mechanisms remain under investigation.
The interaction between HSPBAP1 and HSPB1 influences cellular processes such as cell proliferation, survival, and stress adaptation. Dysregulation of HSPBAP1 has been implicated in diseases like cancer, where it may exhibit dual roles. Some evidence suggests it promotes apoptosis in certain malignancies by interfering with HSPB1’s anti-apoptotic functions, while other studies highlight its oncogenic potential through pathways that enhance cell survival or metastasis. This context-dependent behavior makes HSPBAP1 a complex therapeutic target.
Recombinant HSPBAP1 protein is engineered for in vitro studies to dissect its structural and functional properties. Produced using bacterial or mammalian expression systems, it often includes tags (e.g., His-tag) for purification. Researchers utilize this protein to investigate HSPBAP1-HSPB1 interactions, screen for small-molecule inhibitors, or analyze its role in stress signaling networks. Its recombinant form also aids in structural studies, such as crystallography or NMR, to map binding interfaces critical for chaperone regulation. Further exploration of HSPBAP1 may uncover novel insights into cellular stress responses and disease mechanisms.
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