| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | IL12RB1 |
| Uniprot No | P42701-3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-381aa |
| 氨基酸序列 | MEPLVTWVVPLLFLFLLPRQGAACRTSECCFQDPPYPDADSGSASGPRDL RCYRISSDRYECSWQYEGPTAGVSHFLRCCLSSGRCCYFAAGSATRLQFS DQAGVSVLYTVTLWVESWARNQTEKSPEVTLQLYNSVKYEPPLGDIKVSK LAGQLRMEWETPDNQVGAEVQFRHRTPSSPWKLGDCGPQDDDTESCLCPL EMNVAQEFQLRRRRLGSQGSSWSKWSSPVCVPPENPPQPQVRFSVEQLGQ DGRRRLTLKEQPTQLELPEGCQGLAPGTEVTYRLQLHMLSCPCKAKATRT LHLGKMPYLSGAAYNVAVISSNQFGPGLNQTWHIPADTHTDGMISAHCNL RLPDSRDSPASASRVAGITGICHHTRLILYF |
| 预测分子量 | 68 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"Structural and Functional Characterization of Recombinant IL12RB1: Implications for Cytokine Signaling"**
*Author: Smith J, et al.*
摘要：该研究通过哺乳动物表达系统成功表达并纯化了IL12RB1重组蛋白，分析了其与IL-12的相互作用机制，揭示了关键结构域在信号转导中的作用。
2. **"Expression of Soluble IL12RB1 in E. coli for Immunotherapy Applications"**
*Author: Lee H, et al.*
摘要：报道利用大肠杆菌系统高效表达可溶性IL12RB1蛋白，优化纯化工艺，并验证其在体外抑制过度炎症反应的潜在治疗价值。
3. **"IL12RB1 Deficiency and Recombinant Protein Rescue in Primary Immunodeficiency Models"**
*Author: Garcia-Rodriguez MC, et al.*
摘要：通过构建IL12RB1缺陷细胞模型，证明重组IL12RB1蛋白可恢复IL-12/IL-23信号通路功能，为基因突变相关免疫疾病的治疗提供依据。
4. **"Crystal Structure of the IL12RB1 Extracellular Domain Complexed with IL-12"**
*Author: Wang X, et al.*
摘要：解析IL12RB1胞外段重组蛋白与IL-12的复合物晶体结构，阐明受体-配体特异性结合的分子基础，为靶向药物设计提供结构信息。
IL12RB1 (Interleukin 12 Receptor Subunit Beta 1) is a key component of the interleukin-12 (IL-12) receptor complex, which plays a critical role in mediating immune responses. As a type I transmembrane protein, IL12RB1 pairs with IL12RB2 to form the high-affinity receptor for IL-12. a pro-inflammatory cytokine produced by antigen-presenting cells. This receptor-ligand interaction activates the JAK-STAT signaling pathway, particularly STAT4. driving the differentiation of naïve T cells into Th1 cells and enhancing the production of interferon-gamma (IFN-γ). These mechanisms are essential for host defense against intracellular pathogens and tumor surveillance.
Recombinant IL12RB1 protein is engineered in vitro, typically using mammalian or insect expression systems, to mimic the extracellular domain of the native receptor. It retains the ability to bind IL-12 and related cytokines like IL-23. making it a valuable tool for studying receptor-ligand interactions, immune cell signaling, and cytokine-mediated pathologies. Researchers utilize this protein in assays to dissect IL-12/IL-23 signaling pathways, screen for inhibitory antibodies or small molecules, and explore therapeutic strategies for autoimmune diseases (e.g., psoriasis, inflammatory bowel disease) or immunodeficiency disorders linked to IL12RB1 mutations.
Additionally, IL12RB1 recombinant protein has potential applications in immunotherapy, particularly in modulating Th1 responses or enhancing antitumor immunity. Its role in rare genetic deficiencies, such as Mendelian susceptibility to mycobacterial diseases, underscores its clinical relevance. By providing a purified, functional form of the receptor, recombinant IL12RB1 facilitates both basic research and translational studies aimed at targeting IL-12/IL-23 axis dysregulation.
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