| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | CDC8; TMPK; TYMK; PP3731 |
| WB Predicted band size | 24 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human DTYMK |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于DTYMK抗体的3篇参考文献示例(注：以下内容为模拟生成，实际文献需根据具体研究查询)：
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1. **文献名称**: *Development and Characterization of a Monoclonal Antibody Against Human DTYMK for Cancer Biomarker Studies*
**作者**: Zhang L, et al.
**摘要**: 该研究报道了一种针对人源DTYMK蛋白的单克隆抗体的开发与验证。通过免疫印迹和免疫组化实验，证实该抗体具有高特异性和灵敏度，可用于检测多种癌症组织(如结直肠癌和乳腺癌)中DTYMK的过表达现象，为癌症诊断提供了潜在工具。
2. **文献名称**: *DTYMK Antibody-Based Analysis of Thymidylate Kinase Activity in Cell Cycle Regulation*
**作者**: Tanaka K, et al.
**摘要**: 研究利用DTYMK特异性抗体探究该酶在细胞周期进程中的作用。实验发现DTYMK在S期表达显著升高，并通过siRNA和抗体阻断实验证实其活性对DNA合成至关重要，提示其在细胞增殖调控中的关键地位。
3. **文献名称**: *Immunohistochemical Detection of DTYMK in Glioblastoma: Correlation with Chemotherapy Resistance*
**作者**: Smith J, et al.
**摘要**: 本研究通过商业化DTYMK抗体分析胶质母细胞瘤样本，发现DTYMK高表达与替莫唑胺耐药性相关。研究提示DTYMK可能作为预测化疗反应的生物标志物，并为其机制研究提供实验依据。
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如需实际文献，建议在PubMed或Google Scholar中搜索关键词“DTYMK antibody”或结合具体研究领域筛选。
The DTYMK (deoxythymidylate kinase) antibody is a research tool targeting the enzyme encoded by the DTYMK gene, which plays a critical role in DNA synthesis and repair. DTYMK catalyzes the phosphorylation of deoxythymidine monophosphate (dTMP) to deoxythymidine diphosphate (dTDP), a rate-limiting step in the thymidine nucleotide salvage pathway. This pathway is essential for maintaining balanced dNTP pools required for genome replication and stability. Dysregulation of DTYMK has been implicated in cancer progression, as rapidly proliferating cells rely heavily on nucleotide metabolism.
DTYMK antibodies are primarily used to study protein expression, localization, and function in cellular models or clinical samples. They enable detection via techniques like Western blot, immunohistochemistry, or immunofluorescence. Researchers employ these antibodies to explore DTYMK's role in cancer biology, particularly its association with chemotherapeutic resistance (e.g., 5-fluorouracil) and prognosis. Elevated DTYMK levels in tumors, such as colorectal or gastric cancers, have been linked to poor survival, suggesting its potential as a therapeutic target or biomarker.
Commercial DTYMK antibodies are typically developed using immunogenic peptides or recombinant protein fragments. Validation includes specificity tests (knockdown/knockout controls) and application-specific performance checks. Ongoing studies focus on clarifying its mechanistic contributions to oncogenesis and optimizing antibody reliability for diagnostic or translational applications.
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