| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/30-1/150 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | EPHEXIN; ARHGEF27 |
| WB Predicted band size | 82 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human NGEF |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下为模拟的示例参考文献框架(非真实文献，供参考格式)：
1. **文献名称**: "Development and Characterization of a Novel NGEF-Specific Monoclonal Antibody for Neural Signaling Studies"
**作者**: Chen L, et al.
**摘要**: 该研究报道了一种新型抗NGEF单克隆抗体的开发，验证了其在Western blot和免疫组化中的特异性，证实NGEF在神经元突触中的定位及与Rho GTPase通路的相互作用。
2. **文献名称**: "NGEF Antibody-Based Detection of Neuroguidin in Glioblastoma Progression"
**作者**: Martinez R, et al.
**摘要**: 利用商业化NGEF抗体分析胶质母细胞瘤组织，发现NGEF表达水平与肿瘤侵袭性正相关，提示其作为潜在生物标志物的价值。
3. **文献名称**: "Functional Validation of NGEF Antibodies in Axon Guidance Assays"
**作者**: Kim S, et al.
**摘要**: 通过CRISPR敲除和抗体阻断实验，验证了NGEF抗体在体外轴突导向模型中的功能抑制作用，证明NGEF通过调控细胞骨架重组影响神经发育。
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**注意**：以上文献为示例，实际研究中请通过PubMed、Web of Science等平台以关键词“NGEF antibody”或“Neuroguidin exchange factor antibody”检索最新文献。可尝试结合疾病名称(如“cancer”“neurological disorders”)或技术类型(如“monoclonal”“immunohistochemistry”)缩小搜索范围。
**Background of NGEF Antibody**
Neuronal Guanine Nucleotide Exchange Factor (NGEF), also known as ARHGEF27 or PLEKHG5. belongs to the RhoGEF family, which regulates Rho GTPases—key mediators of cytoskeletal reorganization, cell migration, and signaling pathways. NGEF is predominantly expressed in the nervous system, where it interacts with Rho GTPases like RhoA and Cdc42 to modulate neuronal morphology, axon guidance, and synaptic plasticity. Studies highlight its role in neuronal development, particularly in dendritic spine formation and neurite outgrowth, processes critical for neural network establishment and function.
Dysregulation of NGEF has been implicated in neurological disorders. For instance, mutations in the *PLEKHG5* gene are linked to motor neuron diseases, including autosomal recessive lower motor neuron disease (LMND) and Charcot-Marie-Tooth neuropathy. Altered NGEF expression is also observed in neurodegenerative conditions like Alzheimer’s disease, potentially influencing amyloid-beta-induced synaptic toxicity. Additionally, NGEF may contribute to psychiatric disorders through its impact on synaptic signaling.
NGEF antibodies are essential tools for detecting NGEF protein levels, localization, and activity in research. They enable techniques such as Western blotting, immunohistochemistry, and immunofluorescence, aiding in the exploration of NGEF’s mechanistic roles in health and disease. These antibodies also support drug discovery efforts by identifying NGEF as a potential therapeutic target for neurological conditions. Overall, NGEF antibodies bridge molecular insights with clinical applications, advancing understanding of neural pathophysiology.
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