| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/10000 | Human,Mouse,Rat |
| Aliases | KNTC1AP; hZwilch |
| WB Predicted band size | 67 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human ZWILCH |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于ZWILCH抗体的3篇代表性文献示例(注：以下内容为模拟虚构，仅供参考格式)：
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1. **文献名称**: *ZWILCH, a novel component of the mitotic checkpoint, interacts with kinetochore proteins in human cells*
**作者**: Kops GJ, et al.
**摘要**: 本研究首次报道了ZWILCH蛋白作为人类细胞有丝分裂检查点的组成部分，通过特异性抗体检测其与动粒蛋白的结合。实验表明ZWILCH抗体可有效用于免疫沉淀和免疫荧光，揭示其在染色体正确分离中的调控作用，并与癌症基因组不稳定性相关。
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2. **文献名称**: *Development and validation of a ZWILCH-specific monoclonal antibody for cancer biomarker studies*
**作者**: Smith LM, et al.
**摘要**: 研究团队开发了一种高特异性的ZWILCH单克隆抗体，并通过Western blot和免疫组化验证其可靠性。利用该抗体分析结直肠癌组织样本，发现ZWILCH过表达与患者生存率降低显著相关，提示其作为潜在预后标志物的价值。
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3. **文献名称**: *ZWILCH knockdown impairs DNA damage response: Insights from antibody-based functional assays*
**作者**: Chen R, et al.
**摘要**: 通过siRNA和抗ZWILCH抗体的联合应用，研究发现ZWILCH缺失会导致细胞对DNA损伤的修复能力下降。抗体介导的蛋白定位分析显示，ZWILCH在核内与修复蛋白相互作用，为癌症治疗靶点提供了新思路。
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如需真实文献，建议通过PubMed或Google Scholar搜索“ZWILCH antibody”或“ZWILCH kinetochore”获取具体信息。
The ZWILCH antibody targets the ZWILCH protein, a component of the mitotic checkpoint complex (MCC) critical for proper chromosome segregation during cell division. ZWILCH, also known as Zwilch kinetochore protein, interacts with other checkpoint proteins like ZWINT and Rod to ensure accurate microtubule-kinetochore attachment. It plays a role in the spindle assembly checkpoint (SAC), delaying anaphase until all chromosomes are correctly aligned, thereby preventing aneuploidy—a hallmark of cancer and genetic disorders.
ZWILCH antibodies are essential tools in studying cell cycle regulation, mitotic errors, and chromosomal instability. They are used in techniques like immunofluorescence, Western blotting, and immunohistochemistry to localize ZWILCH in cells or tissues, assess its expression levels, or analyze its interaction partners. Research has linked aberrant ZWILCH expression to tumorigenesis, making it a potential biomarker or therapeutic target in cancers. Commercial ZWILCH antibodies are typically raised in rabbits or mice, validated for specificity across applications.
Understanding ZWILCH's role through antibody-based studies contributes to insights into mitotic fidelity, cancer mechanisms, and developmental disorders caused by chromosomal missegregation.
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