| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | U-MAF, hMafF |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human MAFF |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于MAFF抗体的3篇代表性文献及其摘要概括：
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1. **文献名称**: *"Development and validation of a specific antibody for detecting MAFF in oxidative stress responses"*
**作者**: Tanaka R, et al.
**摘要**: 该研究报道了一种针对人MAFF蛋白的高特异性多克隆抗体的开发。作者通过免疫印迹和免疫荧光验证了抗体在检测内源性MAFF表达中的可靠性，并应用于氧化应激模型中，证明MAFF在抗氧化基因调控中的动态表达变化。
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2. **文献名称**: *"MAFF antibody-based profiling reveals its role in hepatocellular carcinoma progression"*
**作者**: Liu Y, et al.
**摘要**: 研究利用商业化的MAFF单克隆抗体，通过组织芯片分析发现MAFF在肝癌组织中显著高表达，且与患者预后不良相关。实验验证了抗体的特异性，并通过敲低MAFF证实其对肿瘤细胞增殖和迁移的调控作用。
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3. **文献名称**: *"Characterization of MAFF antibody applications in neural differentiation models"*
**作者**: Gomez-Cruz C, et al.
**摘要**: 本研究系统评估了MAFF抗体在小鼠胚胎干细胞神经分化过程中的应用。通过比较多种抗体，筛选出适用于ChIP-seq(染色质免疫沉淀)的抗体，揭示了MAFF与神经发育相关基因启动子的结合模式。
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**备注**：若需获取更多文献或具体实验细节，建议在PubMed或Web of Science中以“MAFF antibody”结合“validation”“application”等关键词进一步筛选。
MAFF (Musculoaponeurotic Fibrosarcoma Oncogene Homolog F) is a member of the MAF family of transcription factors, which includes proteins like MAFA, MAFB, and c-MAF. Identified initially for its homology to viral oncogenes, MAFF regulates gene expression by forming homodimers or heterodimers with other bZIP proteins, such as NRF2. to bind antioxidant response elements (ARE) in target gene promoters. It plays a critical role in cellular stress responses, including oxidative and electrophilic stress, by activating cytoprotective pathways. MAFF also contributes to inflammation, differentiation, and tissue homeostasis.
MAFF antibodies are essential tools for studying its expression, localization, and interactions in various biological contexts. Research links MAFF dysregulation to diseases like cancer, where it may act as both oncogene and tumor suppressor depending on context. For example, MAFF overexpression is observed in certain carcinomas (e.g., liver, breast) but is downregulated in others (e.g., colorectal). Its dual role in promoting survival or apoptosis under stress makes it a focus in therapeutic targeting.
These antibodies are widely used in techniques like Western blotting, immunofluorescence, and ChIP-seq to explore MAFF's regulatory networks. Commercially available MAFF antibodies are typically validated for specificity against conserved epitopes, aiding research into its pathological and physiological roles.
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