| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | JMJD3; NEDCFSA |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human KDM6B |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于KDM6B抗体的研究文献概览：
1. **文献名称**：The histone demethylase KDM6B regulates lineage commitment in early mouse embryos(作者：S. Hoshino等)
**摘要**：该研究通过免疫沉淀和Western blot技术，利用KDM6B特异性抗体验证其在胚胎干细胞分化中的关键作用，发现KDM6B通过去除H3K27me3修饰调控多能性基因表达。
2. **文献名称**：KDM6B promotes TGF-β-induced epithelial-mesenchymal transition in human pancreatic cancer cells(作者：Y. Li等)
**摘要**：研究使用KDM6B抗体进行染色质免疫共沉淀(ChIP)，揭示KDM6B通过激活促转移基因促进胰腺癌侵袭，其表达水平与患者预后显著相关。
3. **文献名称**：Development of a monoclonal antibody specific for the histone demethylase KDM6B(作者：T. Nakamura等)
**摘要**：文章详细描述了针对KDM6B C端结构域的单克隆抗体制备过程，验证了其在免疫荧光和流式细胞术中的高特异性，为表观遗传学研究提供可靠工具。
注：以上为示例性内容，实际文献需通过PubMed等数据库检索确认。
The KDM6B antibody is a crucial tool in epigenetic research, targeting the lysine demethylase 6B (KDM6B), also known as JMJD3. KDM6B is a histone-modifying enzyme belonging to the KDM6 subfamily, which specifically demethylates di- and tri-methylated lysine 27 on histone H3 (H3K27me2/3). This post-translational modification is linked to transcriptional repression, and its removal by KDM6B activates gene expression, playing pivotal roles in development, cellular differentiation, and immune responses. KDM6B is implicated in regulating stem cell pluripotency, somatic cell reprogramming, and senescence.
Antibodies against KDM6B enable the detection and localization of the protein in various experimental applications, including Western blotting, chromatin immunoprecipitation (ChIP), and immunofluorescence. They are widely used to study KDM6B's involvement in diseases such as cancer, where it may act as an oncogene or tumor suppressor depending on context, and neurodevelopmental disorders. Dysregulation of KDM6B has been associated with multiple cancers (e.g., leukemia, glioblastoma), autoimmune diseases, and aging-related pathologies. Researchers also utilize these antibodies to explore its interaction with signaling pathways like Wnt and TGF-β. As an essential reagent, KDM6B antibodies advance understanding of epigenetic mechanisms and potential therapeutic targets in human health and disease.
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