| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/10000 | Human,Mouse,Rat |
| Aliases | KS; KNO; KNO1 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human COL18A1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是与COL18A1抗体相关的3篇参考文献及其简要摘要：
1. **文献名称**：*Collagen XVIII/endostatin structure and functional role in angiogenesis*
**作者**：Marneros AG, Olsen BR
**摘要**：该研究通过COL18A1抗体分析胶原蛋白XVIII的结构及其水解产物内皮抑素(endostatin)的功能，揭示其在抑制肿瘤血管生成中的作用，为抗血管治疗提供理论基础。
2. **文献名称**：*Mutations in COL18A1 cause Knobloch syndrome*
**作者**：Sertié AL, et al.
**摘要**：利用COL18A1抗体检测患者组织样本，发现基因突变导致胶原蛋白XVIII表达异常，与Knobloch综合征(眼部及颅骨发育缺陷疾病)的发病机制密切相关。
3. **文献名称**：*Tissue distribution and activity of collagen XVIII in cancer progression*
**作者**：Fukai N, et al.
**摘要**：通过免疫组化(使用COL18A1抗体)分析多种癌症组织中胶原蛋白XVIII的分布，发现其在肿瘤基质中高表达，可能通过调控细胞黏附影响转移过程。
COL18A1 antibody targets the α1 chain of type XVIII collagen, a basement membrane protein encoded by the COL18A1 gene. This collagen belongs to the multiplexin family, characterized by multiple triple-helix domains and a C-terminal endostatin domain with anti-angiogenic properties. Type XVIII collagen is expressed in various tissues, including vascular endothelium, epithelial layers, and the eye, where it contributes to extracellular matrix (ECM) stability and tissue organization. It plays roles in angiogenesis, cell adhesion, and maintenance of retinal integrity. Mutations in COL18A1 are linked to Knobloch syndrome, a rare autosomal recessive disorder characterized by ocular abnormalities and occipital skull defects.
COL18A1 antibodies are essential tools for studying the protein's localization, expression, and function in physiological and pathological contexts. They are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to investigate collagen XVIII's involvement in diseases such as cancer (via endostatin's tumor-suppressive effects), diabetic retinopathy, and neurodegenerative conditions. Researchers often select antibodies targeting specific domains (e.g., endostatin) to explore functional mechanisms. Validation includes assessing reactivity in knockout models or tissue-specific expression patterns. Commercial antibodies vary by host species (rabbit, mouse), clonality (monoclonal/polyoclonal), and epitope specificity, necessitating careful selection based on experimental goals. Reliable COL18A1 antibodies advance insights into ECM biology and therapeutic targeting of angiogenesis-related diseases.
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