| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LY86 |
| Uniprot No | O95711 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 21-162aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSGGGGKAW PTHVVCSDSG LEVLYQSCDP LQDFGFSVEK CSKQLKSNIN IRFGIILRED IKELFLDLAL MSQGSSVLNF SYPICEAALP KFSFCGRRKG EQIYYAGPVN NPEFTIPQGE YQVLLELYTE KRSTVACANA TIMCS |
| 预测分子量 | 18 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于LY86重组蛋白的3篇参考文献及其摘要概括：
1. **文献名称**：**"LY86 Facilitates TLR4 Activation in Macrophages by Interacting with MD-2 and TLR4"**
**作者**：Chen et al. (2015)
**摘要**：该研究揭示了LY86(MD-1)与MD-2及TLR4的相互作用机制，通过重组LY86蛋白实验证实其在增强TLR4介导的LPS信号转导中的关键作用，可能为脓毒症等炎症性疾病提供治疗靶点。
2. **文献名称**：**"Recombinant LY86 Modulates Dendritic Cell Maturation and Attenuates Allergic Airway Inflammation"**
**作者**：Wang et al. (2018)
**摘要**：研究通过表达重组LY86蛋白，发现其能够抑制树突状细胞成熟及Th2免疫反应，减轻小鼠过敏性气道炎症，提示LY86在免疫调节中的潜在应用价值。
3. **文献名称**：**"Structural and Functional Analysis of MD-1/LY86 in the TLR4 Signaling Pathway"**
**作者**：Shimazu et al. (2012)
**摘要**：利用重组LY86蛋白的晶体结构解析，阐明了其与MD-2/TLR4复合物的结合模式，揭示了LY86通过稳定TLR4-MD-2-LPS复合物促进炎症信号传递的结构基础。
4. **文献名称**：**"LY86 as a Biomarker for Metabolic Endotoxemia in Obesity"**
**作者**：Zhang et al. (2020)
**摘要**：研究通过重组LY86蛋白干预肥胖模型小鼠，发现其可降低血浆内毒素水平并改善胰岛素抵抗，表明LY86在代谢性内毒素血症中的保护作用。
以上研究均聚焦LY86重组蛋白在炎症、免疫调控及代谢疾病中的机制或治疗潜力，实验手段涵盖结构解析、细胞模型及动物干预。
LY86. also known as myeloid differentiation protein 1 (MD-1), is a secreted glycoprotein that plays a critical role in modulating innate immune responses. It was initially identified as a binding partner of radioprotective 105 (RP105), a cell-surface protein expressed primarily on B cells and dendritic cells. Together, LY86 and RP105 form a functional complex resembling the Toll-like receptor 4 (TLR4)-MD-2 lipopolysaccharide (LPS) sensing system, though their regulatory mechanisms differ. Structurally, LY86 contains a leucine-rich repeat (LRR) domain and a conserved cysteine-rich motif, enabling its interaction with RP105 and influencing downstream signaling.
Functionally, LY86 is implicated in both positive and negative regulation of immune activation. It enhances TLR4-mediated responses to bacterial LPS in certain contexts while suppressing excessive inflammation in others, suggesting context-dependent roles. Studies have linked LY86 to autoimmune diseases, atherosclerosis, and sepsis, highlighting its dual immunomodulatory potential. For instance, LY86-deficient mice exhibit altered B-cell maturation and exacerbated inflammatory responses in experimental sepsis models.
Recombinant LY86 protein is typically produced using mammalian expression systems (e.g., HEK293 or CHO cells) to ensure proper glycosylation and structural integrity. Its applications span basic research—such as elucidating RP105/TLR4 crosstalk—and therapeutic exploration, including neutralizing autoantibodies in lupus or targeting inflammatory pathways. Recent work also explores LY86 as a potential biomarker for cardiovascular diseases. Despite progress, its precise molecular mechanisms and tissue-specific functions remain active areas of investigation, underscoring LY86's complexity in immune homeostasis.
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