| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | . |
| Uniprot No | P08659 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-550aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMMEDAKNIKKGPAPFYPLEDGTAGEQLHKA MKRYALVPGTIAFTDAHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIV VCSENSLQFFMPVLGALFIGVAVAPANDIYNERELLNSMGISQPTVVFVS KKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQSMYTFVTSHLPPGFNEY DFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHARDPIFGN QIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQD YKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAK RFHLPGIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTG KTLGVNQRGELCVRGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDED EHFFIVDRLKSLIKYKGYQVAPAELESILLQHPNIFDAGVAGLPDDDAGE LPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVVFVDEVPKGLTGK LDARKIREILIKAKKGGKIAV |
| 预测分子量 | 63 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
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**Background of Recomcombinant Proteins**
Recombinant proteins are engineered molecules produced by introducing specific DNA sequences into host organisms, enabling them to synthesize proteins that are otherwise inaccessible or scarce in nature. This technology emerged in the 1970s following breakthroughs in genetic engineering, particularly the development of recombinant DNA techniques by researchers like Stanley Cohen and Herbert Boyer. By splicing genes into plasmids and transferring them into bacterial, yeast, or mammalian cells, scientists unlocked the ability to mass-produce proteins with high purity and consistency.
The production process typically involves isolating a target gene, inserting it into an expression vector, and introducing the vector into a host organism. Common hosts include *E. coli* (for simple proteins), yeast (for glycosylated proteins), and mammalian cells (for complex post-translational modifications). Advances in bioprocessing, such as high-density fermentation and chromatography-based purification, have significantly enhanced yield and scalability.
Recombinant proteins revolutionized medicine and biotechnology. They underpin therapies like insulin for diabetes, monoclonal antibodies for cancer, and vaccines (e.g., hepatitis B). In research, they serve as tools for studying protein function, signaling pathways, and drug development. Industrial applications range from enzyme production for biofuels to biodegradable plastics.
Challenges persist, including optimizing expression systems for difficult-to-fold proteins, reducing production costs, and minimizing immunogenicity in therapeutic applications. Innovations like CRISPR-Cas9 gene editing, cell-free synthesis, and AI-driven protein design are addressing these hurdles. Today, recombinant protein technology remains a cornerstone of synthetic biology, driving advancements in personalized medicine, sustainable manufacturing, and beyond.
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