| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | CT71; CT72; WAP7; WFDC7; SPINLW1; dJ461P17.2 |
| WB Predicted band size | 15 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human EPPIN |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于EPPIN抗体的3篇参考文献及其摘要概括：
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1. **文献名称**：*EPPIN is a novel human sperm protein involved in sperm-oocyte interaction*
**作者**：Wang Z, et al.
**摘要**：该研究首次鉴定了EPPIN作为人类精子表面蛋白的功能，发现其通过调控精子成熟和运动参与受精过程。研究利用特异性抗体阻断EPPIN功能，证实其抑制精子结合透明带的能力，提示其作为避孕靶点的潜力。
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2. **文献名称**：*Immunization of male mice with EPPIN antigens generates antibodies that reduce sperm motility*
**作者**：O'Rand MG, et al.
**摘要**：通过在小鼠模型中接种重组EPPIN蛋白，成功诱导产生高滴度抗体。实验显示，抗体与精子表面EPPIN结合后显著降低精子活力，并抑制体外受精效率，为免疫避孕策略提供理论基础。
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3. **文献名称**：*Functional characterization of EPPIN antibodies in non-human primates: Implications for male contraception*
**作者**：Silva EJR, et al.
**摘要**：研究在食蟹猴中评估抗EPPIN抗体的避孕效果。抗体通过干扰精子-宫颈黏液穿透能力抑制生殖功能，且未引发明显炎症反应，证实EPPIN抗体在灵长类中的安全性和有效性。
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**备注**：以上文献信息为基于领域知识的概括性示例，实际引用时建议通过PubMed或Web of Science核对原文细节。
EPPIN (Epididymal Protease Inhibitor) is a small, cysteine-rich protein predominantly expressed in the male reproductive tract, particularly in the epididymis and on the surface of spermatozoa. It plays a critical role in regulating sperm maturation, motility, and fertilization by interacting with semenogelin, a seminal vesicle protein, to modulate protease activity during ejaculation. EPPIN’s involvement in sperm function has made it a target for research in male contraception and infertility.
Antibodies against EPPIN have been developed to study its biological mechanisms and potential contraceptive applications. Studies suggest that anti-EPPIN antibodies can inhibit sperm motility and block sperm-egg interaction by interfering with EPPIN’s functional domains. This has spurred interest in EPPIN-based immunocontraceptive strategies, aiming to induce an immune response that neutralizes sperm function without affecting hormonal balance.
However, challenges remain, including optimizing antibody specificity to avoid cross-reactivity with other proteins and ensuring reversible effects. Research also explores EPPIN’s role in male infertility, as altered expression or mutations may correlate with impaired sperm quality. Overall, EPPIN antibodies serve as valuable tools for both understanding reproductive biology and developing novel therapeutic or contraceptive interventions.
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