| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | LC3C; ATG8J |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human MAP1LC3C |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于MAP1LC3C抗体的参考文献及其摘要概括：
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1. **文献名称**: *LC3. a mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processing*
**作者**: Kabeya, Y., et al.
**摘要**: 该研究首次克隆并鉴定了哺乳动物LC3(包括LC3A、LC3B、LC3C亚型)，发现LC3在自噬过程中被蛋白酶切割并定位于自噬体膜，成为自噬的标志性分子工具。文中验证了LC3抗体的特异性，包括针对不同亚型的识别能力。
2. **文献名称**: *Guidelines for the use and interpretation of assays for monitoring autophagy*
**作者**: Klionsky, D.J., et al.
**摘要**: 这篇权威指南总结了自噬研究的关键方法，特别强调了LC3抗体在免疫印迹和免疫荧光中的应用。文中指出LC3C抗体需与其他亚型区分使用，并建议通过基因敲除或siRNA验证抗体的特异性。
3. **文献名称**: *LC3C-mediated autophagy selectively targets the degradation of EMT-related proteins in prostate cancer*
**作者**: Santos, J.C., et al.
**摘要**: 研究利用特异性LC3C抗体发现，LC3C通过选择性自噬降解上皮-间质转化(EMT)相关蛋白，抑制前列腺癌转移。实验验证了抗体在肿瘤组织中的染色特异性及功能研究中的应用。
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**备注**：若需更近期文献，建议在PubMed或Web of Science以“MAP1LC3C antibody”或“LC3C autophagy”为关键词检索，重点关注抗体验证或疾病机制研究。
The MAP1LC3C (microtubule-associated protein 1 light chain 3 gamma) antibody is a crucial tool for studying autophagy, a conserved cellular degradation process. LC3C, a mammalian homolog of yeast Atg8. is one of three LC3 isoforms (LC3A, LC3B, LC3C) involved in autophagosome formation. Unlike LC3A/B, LC3C exhibits distinct functional roles, including selective autophagy pathways and non-canonical autophagy processes such as LC3-associated phagocytosis (LAP) or entosis. It undergoes post-translational lipidation (conversion from LC3C-I to LC3C-II) during autophagosome membrane elongation, serving as a marker for autophagic activity.
LC3C antibodies are widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to detect LC3C expression, localization, and lipidation status. These antibodies help researchers investigate LC3C-specific roles in physiological processes (e.g., immune response, cellular homeostasis) and diseases like cancer, neurodegeneration, and infections. Notably, LC3C is implicated in tumor suppression and immune modulation, making it a therapeutic target in oncology. However, cross-reactivity with other LC3 isoforms must be carefully validated to ensure specificity. High-quality LC3C antibodies are essential for distinguishing its unique functions from those of LC3A/B in autophagy-related studies.
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