| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MAFF |
| Uniprot No | Q9ULX9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-164aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMSVDPLS SKALKIKREL SENTPHLSDE ALMGLSVREL NRHLRGLSAE EVTRLKQRRR TLKNRGYAAS CRVKRVCQKE ELQKQKSELE REVDKLAREN AAMRLELDAL RGKCEALQGF ARSVAAARGP ATLVAPASVI TIVKSTPGSG SGPAHGPDPA HGPASCS |
| 预测分子量 | 20 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
nan
**Background of MAFF Recombinant Protein**
MAFF (Musculoaponeurotic Fibrosarcoma Oncogene Homolog F) is a member of the Maf basic leucine zipper (bZIP) transcription factor family, which includes proteins like MAFA, MAFB, MAFF, and MAFG. These proteins play critical roles in cellular responses to stress, regulation of antioxidant pathways, and control of differentiation and proliferation. MAFF specifically modulates gene expression by forming homodimers or heterodimers with other bZIP proteins, such as Nrf2 (Nuclear factor erythroid 2-related factor 2), to bind antioxidant response elements (ARE) in target gene promoters. This interaction is central to the cellular defense against oxidative stress, inflammation, and xenobiotic metabolism.
Recombinant MAFF protein is engineered using genetic engineering techniques, often expressed in systems like *E. coli*, yeast, or mammalian cells*,* to ensure proper folding and post-translational modifications. It is typically purified via affinity tags (e.g., His-tag or GST-tag) and validated through methods like SDS-PAGE, Western blotting, and functional assays. The recombinant form retains the ability to dimerize and bind DNA, making it invaluable for studying MAFF’s role in redox homeostasis, cellular adaptation to stress, and disease mechanisms (e.g., cancer, neurodegenerative disorders).
Research applications include elucidating MAFF’s dual role as both an oncogene and tumor suppressor, depending on cellular context, and its involvement in the Keap1-Nrf2-ARE pathway. Additionally, MAFF recombinant protein aids in drug discovery, particularly for therapies targeting oxidative stress-related diseases. Its production and characterization have advanced understanding of transcriptional regulation under pathological conditions, offering potential biomarkers or therapeutic targets.
×
