| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MAEA |
| Uniprot No | Q7L5Y9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-396aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMAVQESA AQLSMTLKVQ EYPTLKVPYE TLNKRFRAAQ KNIDRETSHV TMVVAELEKT LSGCPAVDSV VSLLDGVVEK LSVLKRKAVE SIQAEDESAK LCKRRIEHLK EHSSDQPAAA SVWKRKRMDR MMVEHLLRCG YYNTAVKLAR QSGIEDLVNI EMFLTAKEVE ESLERRETAT CLAWCHDNKS RLRKMKSCLE FSLRIQEFIE LIRQNKRLDA VRHARKHFSQ AEGSQLDEVR QAMGMLAFPP DTHISPYKDL LDPARWRMLI QQFRYDNYRL HQLGNNSVFT LTLQAGLSAI KTPQCYKEDG SSKSPDCPVC SRSLNKLAQP LPMAHCANSR LVCKISGDVM NENNPPMMLP NGYVYGYNSL LSIRQDDKVV CPRTKEVFHF SQAEKVYIM |
| 预测分子量 | 48 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
nan
**Background of MAEA Recombinant Protein**
The macrophage erythroblast attacher (MAEA), also known as erythroblast macrophage protein (EMP), is a transmembrane glycoprotein crucial in mediating cell-cell interactions, particularly between erythroblasts and macrophages during erythropoiesis. Discovered in the 1990s, MAEA gained attention for its role in promoting erythroblast enucleation—a key step in red blood cell maturation. Structurally, MAEA contains an extracellular domain with adhesion motifs, a single transmembrane region, and a cytoplasmic tail involved in signaling.
MAEA facilitates the formation of erythroblastic islands (EBIs), specialized niches where erythroblasts interact with central macrophages. These interactions are vital for iron transfer, survival signals, and enucleation. MAEA's extracellular domain binds to receptors on erythroblasts, while its cytoplasmic domain interacts with cytoskeletal proteins like vinculin, anchoring macrophages to the cytoskeleton. This dual binding ensures stable adhesion and coordinated maturation.
Dysregulation of MAEA is linked to hematologic disorders. Mutations or reduced expression can impair erythropoiesis, leading to anemia. Conversely, MAEA overexpression has been observed in certain cancers, where it may promote cell survival or metastasis.
Recombinant MAEA protein is produced using expression systems (e.g., mammalian or insect cells) to retain post-translational modifications. It serves as a research tool to study EBIs, erythropoietic defects, and cell adhesion mechanisms. Additionally, recombinant MAEA holds therapeutic potential, such as in vitro expansion of red blood cells for transfusions or targeting EBIs in blood disorders.
In summary, MAEA is a pivotal protein in erythropoiesis, with recombinant forms enabling deeper exploration of its biologic roles and applications in hematology and beyond.
×
