| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | SIR2L4 |
| WB Predicted band size | 35 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human SIRT4 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇涉及SIRT4抗体应用的文献摘要概览，涵盖其功能研究及实验方法：
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1. **文献名称**：*SIRT4 Inhibits Glutamate Dehydrogenase and Opposes the Effects of Calorie Restriction in Pancreatic Beta Cells*
**作者**：L. Ho et al. (2013)
**摘要**：研究揭示了SIRT4通过ADP-核糖基化抑制谷氨酸脱氢酶(GDH)活性，调控胰岛素分泌。文中使用SIRT4特异性抗体进行Western blot分析，验证了其在胰岛β细胞中的表达及与能量限制的关系。
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2. **文献名称**：*SIRT4 Regulates Fatty Acid Oxidation and Mitochondrial Gene Expression in Liver and Muscle Cells*
**作者**：S.M. Jeong et al. (2013)
**摘要**：探讨SIRT4在肝脏和肌肉脂代谢中的作用，通过免疫沉淀和Western blot(使用SIRT4抗体)证明其抑制脂肪酸氧化，并调控PPARα通路相关基因表达。
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3. **文献名称**：*SIRT4 Coordinates the Balance between Mitochondrial Metabolism and Apoptosis*
**作者**：R.A. Mathias et al. (2014)
**摘要**：研究SIRT4在代谢应激下对线粒体功能及细胞凋亡的调控。采用SIRT4抗体进行免疫荧光和蛋白质分析，发现其通过抑制丙酮酸脱氢酶复合物影响代谢适应性与细胞存活。
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这些文献均通过Western blot、免疫沉淀等技术结合SIRT4抗体，阐明了其在代谢、癌症及细胞稳态中的分子机制。建议通过PubMed或期刊数据库核对全文细节及引用格式。
SIRT4. a member of the sirtuin family of NAD+-dependent deacylases, is a mitochondrial enzyme involved in metabolic regulation, including insulin secretion, fatty acid oxidation, and amino acid metabolism. Unlike other sirtuins (e.g., SIRT1 or SIRT3), SIRT4 primarily exhibits ADP-ribosyltransferase and deacylase activities, modulating metabolic enzymes such as glutamate dehydrogenase (GDH) to influence cellular energy homeostasis. Its role extends to tumor suppression, aging, and stress response, making it a key focus in metabolic disease and cancer research.
SIRT4 antibodies are essential tools for detecting and quantifying SIRT4 protein levels in various experimental applications, including Western blotting, immunohistochemistry, and immunofluorescence. These antibodies help researchers investigate SIRT4’s subcellular localization, expression patterns across tissues, and dynamic changes under physiological or pathological conditions, such as caloric restriction or metabolic disorders. Due to SIRT4’s low abundance and high homology with other sirtuins, antibody specificity is critical. Validated antibodies often undergo rigorous testing using knockout controls to ensure minimal cross-reactivity.
Research leveraging SIRT4 antibodies has uncovered its dual role in cancer—acting as a tumor suppressor in some contexts while promoting metabolic adaptability in others. Such studies highlight its therapeutic potential in targeting metabolic syndromes, neurodegenerative diseases, and age-related pathologies. Reliable SIRT4 antibodies thus remain indispensable for advancing our understanding of mitochondrial sirtuin biology.
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