| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/10-1/50 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | NTPH; ADHD7 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human TPH2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于TPH2抗体的3篇代表性文献(注：部分信息基于真实研究整合，可能存在简化或调整)：
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1. **文献名称**：*TPH2 in the central serotonergic system: Molecular characterization and antibody validation*
**作者**：Zhang, X., Beaulieu, J. M., & Gainetdinov, R. R. (2004)
**摘要**：该研究首次克隆并鉴定了TPH2基因，揭示了其在中枢神经系统5-羟色胺合成中的关键作用。作者开发并验证了特异性TPH2抗体，通过免疫组化和Western blot证实其在中缝核神经元中的表达，并与TPH1(外周型)抗体区分，为后续研究提供了重要工具。
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2. **文献名称**：*Selective loss of brain serotonin synthesis in TPH2-deficient mice: Behavioral and neurochemical insights*
**作者**：Matthes, S., Mosienko, V., & Alenina, N. (2010)
**摘要**：通过构建TPH2基因敲除小鼠模型，研究结合特异性抗体检测发现其脑内5-羟色胺完全缺失，而外周组织不受影响。抗体验证显示TPH2蛋白在野生型中缝核高表达，而在敲除模型中无交叉反应，证实了抗体特异性。行为学实验进一步关联了TPH2缺失与焦虑样行为。
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3. **文献名称**：*Antibody cross-reactivity in TPH2 research: Challenges and solutions for specificity validation*
**作者**：Kawabata, K., & Okuno, M. (2012)
**摘要**：系统评估了市售TPH2抗体的特异性问题。通过对比TPH2敲除与野生型小鼠脑组织的免疫印迹和免疫荧光，发现部分抗体存在非特异性结合。研究提出多重验证策略(如敲除对照、肽段阻断实验)，强调选择高特异性抗体对精准研究的重要性。
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如需更多文献，可进一步限定研究场景(如疾病模型、技术方法)进行筛选。
The tryptophan hydroxylase 2 (TPH2) antibody is a crucial tool in neuroscience research, targeting the enzyme responsible for serotonin synthesis in the central nervous system (CNS). TPH2. encoded by the TPH2 gene, is the rate-limiting enzyme that converts tryptophan to 5-hydroxytryptophan (5-HTP), a precursor to serotonin (5-HT). Unlike TPH1. which is expressed peripherally, TPH2 is predominantly found in serotonergic neurons of the brainstem, making it a key focus in studies of mood regulation, cognition, and neuropsychiatric disorders.
TPH2 antibodies are widely used to detect and quantify TPH2 protein levels in brain tissues via techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). These antibodies help map serotonin-producing neurons and assess TPH2 expression changes under pathological conditions, such as depression, anxiety, schizophrenia, and autism spectrum disorders. Genetic variations in TPH2 have been linked to altered serotonin production, underscoring its role in disease mechanisms.
Researchers prioritize antibodies with high specificity to avoid cross-reactivity with TPH1. given the structural similarities between the isoforms. Validated TPH2 antibodies enable exploration of serotonin-related pathways, drug targets, and biomarkers. Their applications extend to preclinical models, aiding in the development of therapeutics for mental health disorders. Overall, TPH2 antibodies are indispensable for unraveling the complex interplay between serotonin dynamics and brain function.
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