| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | OZF |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human ZNF146 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于ZNF146抗体的参考文献概览：
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1. **文献名称**：*ZNF146/OZF acts as a substrate recognition component of Cul2-based E3 ligase complex for ubiquitination of GSK3β*
**作者**：Zhang Y. et al.
**摘要**：研究揭示了ZNF146通过Cul2-E3连接酶复合物泛素化GSK3β的分子机制，使用ZNF146抗体进行免疫共沉淀(Co-IP)验证其与Cul2的相互作用，表明其在Wnt/β-catenin信号通路调控中的作用。
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2. **文献名称**：*PARP-binding zinc finger protein (ZNF146) interacts with the nucleosome through histones and DNA*
**作者**：Kameoka M. et al.
**摘要**：通过染色质免疫沉淀(ChIP)和免疫荧光技术，利用ZNF146抗体证实其通过与组蛋白H3和DNA结合参与核小体重构，并调控PARP1在DNA损伤修复中的功能。
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3. **文献名称**：*Expression and functional analysis of ZNF146 in colorectal cancer progression*
**作者**：Li S. et al.
**摘要**：通过免疫组化(IHC)和Western blotting分析ZNF146在结直肠癌中的表达，发现其过表达与肿瘤转移相关，抗体实验表明ZNF146通过调控EMT通路促进癌细胞侵袭。
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(注：以上文献信息为模拟示例，实际引用需核对具体文献。)
ZNF146 (Zinc Finger Protein 146), also known as OZF or ZKSCAN14. is a member of the Krüppel-associated box (KRAB) domain-containing zinc finger protein family. These proteins typically function as transcriptional regulators, often involved in chromatin remodeling and gene expression control through interactions with specific DNA sequences. ZNF146 contains multiple C2H2-type zinc finger motifs and a KRAB domain, which mediates protein-protein interactions, particularly with co-repressors like KAP1/TIF1β to recruit histone-modifying complexes for transcriptional repression.
Antibodies targeting ZNF146 are essential tools for studying its expression, localization, and molecular interactions. Research has linked ZNF146 to diverse cellular processes, including DNA damage response, cell cycle regulation, and oncogenesis. It interacts with PARP-1 (poly-ADP-ribose polymerase 1), suggesting a role in DNA repair pathways. Additionally, ZNF146 overexpression has been observed in certain cancers, such as hepatocellular carcinoma and breast cancer, where it may promote tumor progression by modulating cell proliferation or apoptosis-related genes.
Commercial ZNF146 antibodies are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to assess protein levels in cell lines and tissue samples. Validation often includes knockout/knockdown controls to confirm specificity. Studies using these antibodies have helped elucidate ZNF146's nuclear localization and its dynamic expression during cell stress or differentiation. Ongoing research aims to clarify its precise mechanistic roles and potential as a therapeutic target, particularly in cancer biology and genome stability maintenance.
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