| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human COL15A1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于COL15A1抗体的参考文献概览：
1. **标题**: "Collagen XV promotes extracellular matrix dynamics and controls angiogenesis"
**作者**: Myers JC等
**摘要**: 研究利用COL15A1抗体揭示胶原XV在血管基底膜重塑中的作用，证明其通过调节内皮细胞迁移影响血管生成，并参与组织修复过程。
2. **标题**: "COL15A1 suppresses malignancy by targeting tumor-stroma interactions in triple-negative breast cancer"
**作者**: Elamaa H等
**摘要**: 通过COL15A1抗体检测发现，胶原XV在乳腺癌微环境中抑制肿瘤生长，其缺失导致基质细胞信号异常并加速肿瘤转移。
3. **标题**: "Proteolytic processing of collagen XV enhances cell adhesion and migration"
**作者**: Aikio M等
**摘要**: 研究采用COL15A1抗体分析胶原XV的酶切片段，发现其C端片段通过整合素信号通路促进细胞黏附，影响肌肉再生与纤维化过程。
注：以上文献为示例性概括，实际引用需核对具体论文年份及期刊来源。
The COL15A1 antibody targets collagen type XV alpha 1 chain, a component of basement membrane zones (BMZs) critical for tissue integrity. Encoded by the COL15A1 gene, this collagen is structurally unique, featuring a large N-terminal non-collagenous domain (NC1) and a shorter collagenous domain. It is expressed in various tissues, including blood vessels, skeletal muscle, and cardiac muscle. Collagen XV supports endothelial cell adhesion, stabilizes microvessels, and modulates extracellular matrix (ECM) organization. Its proteolytic cleavage releases endostatin-like fragments with anti-angiogenic properties, implicating roles in vascular homeostasis and tumor suppression.
COL15A1 antibodies are vital tools for studying collagen XV's distribution and function. They are used in techniques like immunohistochemistry (IHC), immunofluorescence (IF), and Western blotting to visualize tissue-specific expression patterns or quantify protein levels. Research applications include investigating ECM remodeling in diseases such as cancer, muscular dystrophy, and cardiovascular disorders. Mutations in COL15A1 are linked to inherited connective tissue diseases, driving interest in its pathological mechanisms. Commercially available antibodies are typically validated for specificity against human, mouse, or rat collagen XV, with monoclonal and polyclonal variants offering flexibility for experimental needs. Proper validation via knockout controls ensures accuracy in detecting this structurally complex protein.
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