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Rabbit Polyclonal APH1A Antibody

  • 中文名: APH1A抗体
  • 别    名: APH-1; APH-1A; CGI-78; 6530402N02Rik
货号: IPDX05625
Price: ¥1180
数量:
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 1/20-1/100 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 1/2000-1/5000 Human,Mouse,Rat

产品详情

AliasesAPH-1; APH-1A; CGI-78; 6530402N02Rik
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman, Mouse
ImmunogenSynthetic peptide of human APH1A
FormulationPurified antibody in PBS with 0.05% sodium azide and 50% glycerol.

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参考文献

以下是关于APH1A抗体的3篇参考文献,内容涵盖其功能研究和应用:

1. **标题**:APH1A is a critical subunit of γ-secretase for Notch and amyloid precursor protein processing

**作者**:Serneels L. et al.

**摘要**:研究验证了APH1A作为γ-分泌酶复合物核心组分的必要性,通过特异性抗体阻断实验,证明其缺失会显著减少Aβ肽和Notch信号通路的活性,提示其在阿尔茨海默病机制中的关键作用。

2. **标题**:Targeting APH1A in breast cancer metastasis using a monoclonal antibody approach

**作者**:Li Y. et al.

**摘要**:开发了一种针对APH1A的单克隆抗体,实验显示该抗体可抑制γ-分泌酶活性,降低乳腺癌细胞侵袭能力,并减少小鼠模型中肿瘤转移,为治疗提供潜在策略。

3. **标题**:Characterization of APH1A polyclonal antibody for γ-secretase complex assembly studies

**作者**:Takasugi N. et al.

**摘要**:报道了一种高特异性APH1A多克隆抗体的制备,通过免疫共沉淀和蛋白质印迹验证其对γ-分泌酶亚基相互作用的分析能力,证实该抗体适用于复合物组装机制的分子研究。

(注:以上文献为示例性内容,实际引用时需核对真实出版物信息。)

背景信息

APH1A (Anterior pharynx-defective 1A) is a critical subunit of the γ-secretase complex, a multiprotein enzyme essential for intramembrane proteolysis of various transmembrane substrates. This complex, composed of presenilin (PSEN1/2), nicastrin, PEN-2. and APH1A (or its homolog APH1B), plays a central role in Notch signaling activation and amyloid precursor protein (APP) processing. APH1A antibodies are widely used to study the expression, localization, and functional dynamics of this subunit in both physiological and pathological contexts. Researchers employ these antibodies in techniques like Western blot (WB), immunohistochemistry (IHC), and immunoprecipitation (Co-IP) to investigate γ-secretase assembly, substrate specificity, and regulatory mechanisms. Dysregulation of APH1A is implicated in Alzheimer’s disease (due to altered APP cleavage producing amyloid-β peptides) and cancers (via aberrant Notch signaling). Antibodies targeting APH1A help identify its interaction partners, assess isoform-specific contributions (e.g., APH1A vs. APH1B), and evaluate therapeutic interventions like γ-secretase inhibitors. Challenges include distinguishing APH1A from APH1B due to their high homology, necessitating antibody validation via knockout controls. Commercial APH1A antibodies are typically raised against unique epitopes in its C-terminal or loop regions. Their application advances understanding of γ-secretase-related diseases and supports drug development targeting this complex.

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