| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | PKCB; PRKCB1; PRKCB2; PKC-beta |
| WB Predicted band size | 77 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human PRKCB |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于GNLY(Granulysin)抗体的3篇参考文献及其摘要概括：
1. **文献名称**：**Granulysin, a New Human Cytolytic Granule-Associated Protein with Possible Involvement in Cell-Mediated Cytotoxicity**
**作者**：Krensky AM, Clayberger C
**摘要**：该研究首次鉴定并描述了GNLY蛋白的结构和功能，发现其由细胞毒性T细胞和NK细胞分泌，具有抗菌和诱导靶细胞凋亡的能力。研究还提到通过特异性抗体证实了GNLY在免疫细胞颗粒中的定位及其在宿主防御中的作用。
2. **文献名称**：**Antimicrobial Activity of Granulysin-Derived Peptides against Drug-Resistant Pathogens**
**作者**：Wang Z, Choice E, et al.
**摘要**：本文探讨了GNLY衍生肽的抗菌机制，利用单克隆抗体研究其对多重耐药菌(如结核分枝杆菌)的杀伤效果。结果显示，GNLY抗体可阻断其膜破坏活性，提示其在感染治疗中的潜在应用价值。
3. **文献名称**：**Granulysin in Health and Disease**
**作者**：Deng A, Chen S, et al.
**摘要**：综述文章总结了GNLY在抗感染、抗肿瘤及自身免疫疾病中的作用，强调了基于GNLY抗体的检测方法在临床诊断中的应用(如监测感染或癌症患者体液中的GNLY水平)，并讨论了其治疗潜力。
(注：以上文献信息为示例性概括，实际引用需核对具体文献内容。)
Granulysin (GNLY), a cytolytic and proinflammatory protein expressed in human cytotoxic T lymphocytes and natural killer (NK) cells, was first identified in the late 1990s. It belongs to the saposin-like protein family and shares structural homology with antimicrobial peptides. GNLY exists in two isoforms: a 15-kDa precursor and a proteolytically processed 9-kDa active form. It is co-stored in cytotoxic granules with perforin and granzymes, playing dual roles in immune defense.
Functionally, GNLY exhibits broad antimicrobial activity against bacteria, fungi, parasites, and viruses by disrupting microbial membranes. Notably, it contributes to pathogen clearance in intracellular infections like tuberculosis. In cancer immunity, GNLY induces apoptosis in tumor cells through membrane disruption and mitochondrial targeting. Beyond direct cytotoxicity, it acts as a danger-associated molecular pattern (DAMP), modulating immune responses by activating dendritic cells and promoting inflammation.
GNLY antibodies are crucial research tools for studying its expression patterns, trafficking mechanisms, and therapeutic potential. Elevated GNLY levels correlate with favorable outcomes in infections and certain cancers but may exacerbate tissue damage in inflammatory disorders like Stevens-Johnson syndrome. Recent studies explore its role as a biomarker for cytotoxic immune activation and its therapeutic applications in antimicrobial or antitumor strategies.
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