Rabbit Polyclonal E2F3 Antibody

The E2F3 antibody is a crucial tool in studying the E2F3 transcription factor, a member of the E2F family that regulates cell cycle progression, DNA repair, and apoptosis. E2F3 exists as two isoforms, E2F3a and E2F3b, generated via alternative promoters. E2F3a acts as a potent transcriptional activator, driving the expression of genes required for G1/S phase transition and S phase entry, while E2F3b may have repressive or context-dependent roles. Dysregulation of E2F3 is linked to cancer, with overexpression observed in tumors like bladder, ovarian, and prostate cancers, often correlating with poor prognosis. The E2F3 antibody specifically detects E2F3 isoforms, enabling researchers to analyze their expression patterns, subcellular localization, and interactions with other proteins (e.g., retinoblastoma protein, Rb) in normal and diseased tissues. It is widely used in techniques such as Western blotting, immunohistochemistry, and immunofluorescence. Due to high homology among E2F family members, antibody specificity is critical; validated E2F3 antibodies target unique epitopes, often in the C-terminal or N-terminal regions, to minimize cross-reactivity. Research utilizing E2F3 antibodies has advanced understanding of its dual roles in promoting proliferation and suppressing differentiation, as well as its potential as a therapeutic target in oncology.