| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/40-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | FFR; ANG2; ANG3; C11orf2; C11orf3 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human VPS51 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇涉及VPS51抗体的文献摘要信息(基于公开研究整理，部分为模拟示例)：
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1. **文献名称**: *VPS51 regulates lysosomal function through mediating membrane tethering in neuronal cells*
**作者**: Kim J, et al.
**摘要**: 本研究利用VPS51特异性抗体，通过免疫荧光和Western blot分析，揭示了VPS51在神经元溶酶体膜融合中的作用，并发现其缺失导致溶酶体功能异常，可能与神经退行性疾病相关。
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2. **文献名称**: *The role of VPS51 in endosomal-lysosomal trafficking: Insights from CRISPR-Cas9 knockout models*
**作者**: Rodriguez A, et al.
**摘要**: 作者通过构建VPS51敲除细胞系，结合抗体验证蛋白表达缺失，证明VPS51作为GARP复合体成员调控内体-高尔基体逆向运输，其缺失引发溶酶体酶分选缺陷。
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3. **文献名称**: *Antibody-based profiling of VPS51 reveals tissue-specific expression patterns in mammalian development*
**作者**: Chen L, et al.
**摘要**: 该研究开发了高特异性VPS51多克隆抗体，通过免疫组化分析发现VPS51在小鼠胚胎发育中高表达于肝脏和脑组织，提示其在器官形成中的潜在作用。
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**备注**：以上文献为示例性质，实际文献需通过PubMed/Google Scholar等平台以“VPS51 antibody”“GARP complex”“VPS51 function”等关键词检索获取最新数据。建议结合抗体品牌(如Abcam、Sigma等)的产品引用文献提高针对性。
The VPS51 antibody is a tool used to detect VPS51 (vacuolar protein sorting-associated protein 51), a key component of the CORVET (Class C core vacuole/endosome tethering) and GARP (Golgi-associated retrograde protein) complexes. These complexes mediate membrane trafficking, endosomal maturation, and lysosomal targeting by regulating vesicle tethering and fusion. VPS51. along with VPS52. VPS53. and VPS54. forms the GARP complex, which facilitates retrograde transport from endosomes to the trans-Golgi network. Dysregulation of VPS51 is linked to cellular processes like autophagy, neurotransmitter release, and receptor recycling, implicating it in neurodegenerative diseases, cancer, and lysosomal storage disorders.
Researchers use VPS51 antibodies in techniques such as Western blotting, immunofluorescence, and immunoprecipitation to study its expression, localization, and interactions. Commercial VPS51 antibodies are typically raised in rabbits or mice, with validation in knockout models to ensure specificity. Studies have highlighted its role in maintaining organelle integrity and cellular homeostasis, making it a focus in membrane trafficking research. Understanding VPS51's function through antibody-based assays contributes to insights into disease mechanisms and potential therapeutic targets.
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