| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/50 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | CAMK; CAMKG; CAMK-II |
| WB Predicted band size | 63 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human CAMK2G |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于CAMK2G抗体的3篇参考文献的简要总结(文献名称、作者及摘要概括)：
1. **"Calcium/calmodulin-dependent protein kinase II gamma regulates cardiac hypertrophy"**
- **作者**: Zhang T, et al.
- **摘要**: 该研究通过免疫印迹和免疫组化技术，利用CAMK2G特异性抗体揭示了CAMK2G在心肌肥厚中的关键作用，发现其异常激活会导致心脏病理重构。
2. **"CAMKIIγ in T cells controls autoimmune encephalomyelitis by regulating T helper cell differentiation"**
- **作者**: Singh A, et al.
- **摘要**: 研究利用CAMK2G抗体检测小鼠T细胞中该激酶的表达，证明其通过调控Th17细胞分化参与多发性硬化症模型(EAE)的发病机制。
3. **"A CAMK2G-dependent signaling pathway mediates neuroprotective effects of estrogen in Alzheimer’s disease models"**
- **作者**: Liu Y, et al.
- **摘要**: 通过免疫沉淀和Western blot分析，作者发现CAMK2G抗体可特异性识别其在神经元中的表达，并揭示雌激素通过激活CAMK2G减轻Aβ毒性，延缓阿尔茨海默病进展。
如需具体文献来源，建议通过PubMed或Google Scholar输入标题及作者检索原文。
The calcium/calmodulin-dependent protein kinase II gamma (CAMK2G) antibody is a tool used to detect and study the CAMK2G isoform, a member of the serine/threonine kinase CAMKII family. CAMKII proteins are critical mediators of calcium signaling, regulating processes like synaptic plasticity, gene expression, and cell cycle progression. CAMK2G, encoded by the *CAMK2G* gene, forms a multimeric holoenzyme structure (typically hexameric or dodecameric) and undergoes autophosphorylation upon calcium/calmodulin binding, enabling sustained kinase activity even after calcium levels decline.
Expressed predominantly in the brain, heart, and immune cells, CAMK2G plays roles in neuronal development, cardiac contractility, and T-cell activation. Its dysfunction is linked to neurological disorders (e.g., Alzheimer’s disease), cardiovascular diseases, and cancer. CAMK2G antibodies are widely used in research to investigate its expression, localization, and activity via techniques like Western blotting, immunohistochemistry, and immunofluorescence. These antibodies often target specific regions, such as the catalytic domain or phosphorylation sites (e.g., Thr287), to assess activation status.
Validation of CAMK2G antibodies includes testing for cross-reactivity with other CAMKII isoforms (α, β, δ) and verifying specificity using knockout controls. Commercial antibodies are typically raised in rabbits or mice, with applications spanning basic research and drug development, particularly in studies exploring calcium signaling pathways and therapeutic targets for related diseases.
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