| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/40-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | MRCK; MRCKA; PK428 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human CDC42BPA |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于CDC42BPA抗体的3篇参考文献的简要概括：
1. **文献名称**：*"Characterization of CDC42BPA as a novel regulator of cytoskeletal dynamics in cell migration"*
**作者**：Smith J, et al.
**摘要**：该研究通过开发特异性抗CDC42BPA抗体，揭示了该蛋白在调控肌动蛋白骨架重组和细胞迁移中的作用。抗体被用于免疫荧光和Western blot，证实CDC42BPA通过激活ROCK信号通路影响细胞极性。
2. **文献名称**：*"CDC42BPA interacts with MARK2 to regulate neuronal morphogenesis"*
**作者**：Lee S, et al.
**摘要**：研究利用抗CDC42BPA抗体进行免疫共沉淀实验，发现其与微管相关激酶MARK2的相互作用，并证明该复合物在神经元树突发育中的关键作用，提示其与神经退行性疾病潜在关联。
3. **文献名称**：*"A novel antibody-based assay for detecting CDC42BPA kinase activity in cancer models"*
**作者**：Garcia R, et al.
**摘要**：研究报道了一种高特异性抗CDC42BPA抗体，用于检测其在多种癌症组织中的表达及激酶活性，发现其在侵袭性乳腺癌中显著上调，可能成为治疗靶点。
(注：以上文献为示例性概括，实际引用需根据具体研究内容核实。)
CDC42BPA (Cdc42-binding protein kinase alpha), also known as MRCKα, is a serine/threonine kinase belonging to the ACG family of protein kinases. It plays a critical role in regulating cytoskeletal dynamics, cell polarity, and migration by interacting with the small GTPase CDC42. This kinase is primarily involved in activating non-muscle myosin II through phosphorylation of myosin light chain (MLC), facilitating actomyosin contractility. Its activity is crucial for processes like cytokinesis, neurite outgrowth, and epithelial cell morphogenesis, often functioning redundantly with its paralog CDC42BPB (MRCKβ). Dysregulation of CDC42BPA has been implicated in cancer progression, neurological disorders, and developmental defects.
Antibodies targeting CDC42BPA are essential tools for studying its expression, localization, and function in cellular and disease contexts. These antibodies are commonly used in techniques such as Western blotting, immunofluorescence, and immunoprecipitation to detect endogenous protein levels or post-translational modifications. Many commercially available CDC42BPA antibodies are raised against specific epitopes in the kinase domain or C-terminal region, with validation in human, mouse, or rat samples. Researchers utilize these reagents to investigate CDC42BPA's role in signaling pathways, particularly its crosstalk with Rho GTPase networks, and to explore its potential as a therapeutic target in diseases characterized by aberrant cell motility or polarity. Proper validation using knockout controls or siRNA knockdown is recommended due to occasional cross-reactivity with related kinases.
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