| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | CIR |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human CIR1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇与CIR1抗体相关的文献信息(基于公开数据模拟，非真实文献)：
1. **文献名称**：CIR1 interacts with RPA1 to regulate DNA replication and repair
**作者**：Smith J, et al.
**摘要**：本研究通过免疫共沉淀和Western blot技术，利用CIR1抗体验证了CIR1蛋白与复制蛋白A(RPA1)的相互作用，揭示了CIR1在DNA损伤修复和复制压力响应中的关键功能。
2. **文献名称**：CIR1 overexpression promotes tumorigenesis by impairing genomic stability
**作者**：Zhang L, et al.
**摘要**：通过免疫组化(IHC)结合CIR1抗体，研究发现CIR1在多种癌症中异常高表达，其过表达导致染色体不稳定性和肿瘤进展，提示CIR1可作为潜在癌症治疗靶点。
3. **文献名称**：Development and validation of a high-specificity monoclonal antibody for CIR1 detection
**作者**：Kim S, et al.
**摘要**：本文报道了一种新型CIR1单克隆抗体的制备与验证，该抗体在ELISA、免疫荧光和流式细胞术中表现出高灵敏度和特异性，为CIR1相关分子机制研究提供了可靠工具。
4. **文献名称**：CIR1 deficiency disrupts circadian rhythm via transcriptional dysregulation
**作者**：Wang Y, et al.
**摘要**：利用CIR1抗体进行蛋白质表达分析，发现CIR1缺失会干扰生物钟相关基因(如BMAL1)的转录调控，揭示了CIR1在维持昼夜节律中的新功能。
**注**：以上文献为示例性内容，实际研究中请通过PubMed或Google Scholar以“CIR1 antibody”或“CIR1 protein function”等关键词检索真实文献。
CIR1 (Crossover junction recognition protein 1), also known as C1orf177 or Holliday junction recognition protein (HJURP), is a key protein involved in DNA repair and genome stability. It specifically recognizes and binds to Holliday junctions, which are critical intermediates in homologous recombination repair (HRR) during DNA double-strand break (DSB) repair. CIR1 plays a vital role in facilitating the resolution of these structures, ensuring proper chromosome segregation and preventing genomic instability. Additionally, CIR1 is essential for the assembly of the kinetochore, a protein complex that mediates chromosomal attachment to the mitotic spindle during cell division.
Antibodies targeting CIR1 are widely used in molecular and cellular biology research to study its function in DNA repair mechanisms, chromosome dynamics, and cell cycle regulation. These antibodies enable the detection and localization of CIR1 in various experimental setups, including immunofluorescence, Western blotting, and chromatin immunoprecipitation (ChIP). Dysregulation of CIR1 has been implicated in cancer, particularly in tumors with defective DNA repair pathways, making it a potential biomarker or therapeutic target. Research utilizing CIR1 antibodies has provided insights into its role in carcinogenesis, response to DNA-damaging therapies, and mechanisms underlying chromosomal instability, contributing to advancements in oncology and genome integrity studies.
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