| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human CAMSAP1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3-4条关于CAMSAP1抗体的参考文献及其简要摘要：
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1. **文献名称**: "CAMSAP1 regulates microtubule dynamics and neuronal morphology"
**作者**: Jiang et al. (2014)
**摘要**: 该研究利用特异性CAMSAP1抗体，通过免疫荧光和Western blot分析，揭示了CAMSAP1在神经元中稳定微管负端的作用，并证明其缺失会导致神经元轴突形态异常。
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2. **文献名称**: "Structural basis of CAMSAP1 interaction with microtubules"
**作者**: Atherton et al. (2017)
**摘要**: 作者通过冷冻电镜和CAMSAP1抗体标记，解析了CAMSAP1与微管结合的分子机制，发现其通过N端结构域锚定微管负端以维持细胞极性。
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3. **文献名称**: "CAMSAP1 deficiency disrupts intestinal epithelial cell polarity"
**作者**: Toya et al. (2016)
**摘要**: 研究使用CAMSAP1抗体进行免疫组化，发现CAMSAP1缺失导致肠道上皮细胞微管排列紊乱，影响细胞极性和屏障功能，提示其在组织稳态中的关键作用。
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4. **文献名称**: "CAMSAP1 is a target of the E3 ubiquitin ligase TRIM21 in cancer cell invasion"
**作者**: Wang et al. (2020)
**摘要**: 该文献通过CAMSAP1抗体结合质谱分析，发现CAMSAP1在肿瘤细胞中受TRIM21调控降解，其表达水平与癌细胞迁移能力呈负相关。
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以上文献均涉及CAMSAP1抗体的实验应用(如定位、功能验证或疾病关联)，覆盖细胞生物学、结构研究及肿瘤学领域。如需具体文献链接或补充信息，可进一步提供。
CAMSAP1 (Calmodulin-regulated Spectrin-associated Protein 1) is a member of the CAMSAP/Patronin family, which plays a critical role in organizing microtubule networks by stabilizing their minus ends and anchoring them to non-centrosomal sites. Unlike centrosomal microtubule-organizing centers, CAMSAP1 regulates microtubule dynamics in polarized cells, influencing cell shape, motility, and intracellular transport. Antibodies targeting CAMSAP1 are essential tools for studying its localization, expression, and interaction with cytoskeletal components.
CAMSAP1 antibodies are widely used in techniques like immunofluorescence, Western blotting, and immunoprecipitation to explore its role in cellular processes such as mitotic spindle orientation, neuronal development, and epithelial cell polarization. They help identify CAMSAP1’s involvement in diseases linked to microtubule dysfunction, including neurodegenerative disorders and cancer.
Most CAMSAP1 antibodies are raised against specific epitopes, often within its conserved N-terminal domain, and are validated for species reactivity (e.g., human, mouse, rat). Researchers prioritize antibodies with high specificity, often verified via knockout cell lines or siRNA-mediated depletion. Commercial CAMSAP1 antibodies are typically available as monoclonal or polyclonal formats, with rabbit and mouse being common host species.
Understanding CAMSAP1’s regulatory mechanisms through these antibodies has advanced insights into cytoskeletal biology, making them indispensable for studies on cell architecture, division, and disease mechanisms.
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