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Mouse CXC-chemokine ligand 16,CXCL16 ELISA Kit

  • 中文名: 小鼠CXC趋化因子配体16(CXCL16)ELISA Kit
货号: IPD-E08873m
Price: ¥2500
数量:
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产品详情

  • 产品描述:
    小鼠CXC趋化因子配体16(CXCL16)酶联免疫试剂盒检测血清、血浆,组织匀浆样本中的CXCL16含量。CXCL16是重要靶点。背景上,它在免疫调节等生理过程中有作用,且与多种疾病发展相关。研究机制方面,其与受体结合介导细胞间信号传导,参与炎症反应、肿瘤细胞迁移等,为相关疾病治疗提供潜在方向。试剂盒检测范围为7.8125 pg/mL-500 pg/mL,灵敏度为3.012 pg/mL。适用于科研场景下炎症相关动物模型构建、药物干预效果评估或免疫调控机制研究,特别针对心血管疾病、自身免疫性疾病等领域的实验需求,能够稳定检测低丰度CXCL16分子,为探索其生物学功能提供可靠数据支持。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
  • 别名:
    Cxcl16 ELISA Kit; SrpsoxC-X-C motif chemokine 16 ELISA Kit; Scavenger receptor for phosphatidylserine and oxidized low density lipoprotein ELISA Kit; SR-PSOX ELISA Kit; Small-inducible cytokine B16 ELISA Kit; Transmembrane chemokine CXCL16 ELISA Kit
  • 缩写:
    CXCL16
  • Uniprot No.:
    Q8BSU2
  • 种属:
    Mus musculus (Mouse)
  • 样本类型:
    serum, plasma,tissue homogenates
  • 检测范围:
    7.8125 pg/mL-500 pg/mL
  • 灵敏度:
    3.012 pg/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Immunology
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



     Intra-Assay PrecisionInter-Assay PrecisionSample123123n202020202020Mean(pg/ml)56.74257.87660.12658.66659.90261.355SD0.0320.0280.0310.0290.0320.036CV(%)6.0265.1765.5265.2925.7256.294
  • 线性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of mouse CXCL16 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.




    Sample

    Serum(n=4)



    1:1

    Average %

    89



    Range %

    83-95



    1:2

    Average %

    102



    Range %

    96-108



    1:4

    Average %

    99



    Range %

    93-105



    1:8

    Average %

    92



    Range %

    86-98










  • 回收率:

    The recovery of mouse CXCL16 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.



    Sample Type

    Average % Recovery

    Range



    Serum (n=5)

    88

    82-94



    EDTA plasma (n=4)

    95

    90-101










    SAMPLE VALUES

    Serum/Plasma - Samples from apparently healthy volunteers were evaluated for the presence of mouse CXCL16 in this assay. No medical histories were available for the donors used in this study.

    Sample TypeMean (pg/mL)Range (pg/mL)Serum (n=70)702554-1002EDTA plasma (n=45)689401-902Heparin plasma (n=35)722504-1186

  • 标准曲线:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.








    pg/ml

    OD1

    OD2

    Average

    Corrected


    500

    2.531

    2.585

    2.558

    2.450


    250

    1.959

    1.945

    1.952

    1.844


    125

    1.213

    1.180

    1.196

    1.088


    62.5

    0.516

    0.521

    0.519

    0.411


    31.25

    0.344

    0.354

    0.349

    0.241


    15.625

    0.226

    0.235

    0.231

    0.123


    7.8125

    0.156

    0.168

    0.162

    0.054


    0

    0.113

    0.103

    0.108










  • 货期:
    3-5 working days

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