| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | CK; EK; CHK; CKI |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human CHKA |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于CHKA(胆碱激酶α)抗体的代表性文献概览：
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1. **文献名称**：*Choline kinase alpha as a therapeutic target in cancer*
**作者**：Glunde K et al.
**摘要**：该研究探讨了CHKA在肿瘤代谢中的关键作用，开发了特异性CHKA抗体用于检测其在乳腺癌和前列腺癌中的过表达。通过免疫组化和Western blot验证，发现CHKA高表达与患者预后不良相关，提示其作为潜在治疗靶点。
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2. **文献名称**：*Targeting choline kinase α in colorectal cancer*
**作者**：Ramírez de Molina A et al.
**摘要**：研究利用CHKA特异性抗体分析结直肠癌组织中CHKA蛋白水平，发现其表达与肿瘤侵袭性正相关。通过抗体阻断实验证实，抑制CHKA活性可降低癌细胞增殖和迁移能力，支持其在靶向治疗中的应用。
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3. **文献名称**：*Development of a monoclonal antibody against human choline kinase α*
**作者**：Hernández-Alcoceba R et al.
**摘要**：该文献报道了一种新型CHKA单克隆抗体的开发及验证，证明其在ELISA、免疫荧光和流式细胞术中的高特异性和灵敏度。抗体成功应用于筛选CHKA抑制剂，为药物研发提供工具。
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4. **文献名称**：*CHKA regulates EMT and metastasis in non-small cell lung cancer*
**作者**：Li Y et al.
**摘要**：研究采用CHKA抗体检测非小细胞肺癌(NSCLC)中CHKA的表达，发现其通过调控上皮-间质转化(EMT)促进转移。敲低CHKA后，EMT标志物表达下降，提示其作为抑制转移的潜在靶点。
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以上文献展示了CHKA抗体在疾病机制研究、治疗靶点验证及药物开发中的关键作用。如需具体文献来源，可进一步通过PubMed或期刊数据库检索。
CHKA (Choline Kinase Alpha) is a key enzyme in phospholipid metabolism, catalyzing the phosphorylation of choline to phosphocholine, the first step in the synthesis of phosphatidylcholine—a major component of cell membranes. It plays a critical role in maintaining membrane integrity, cell signaling, and lipid homeostasis. Dysregulation of CHKA has been linked to cancer, where its overexpression is observed in various malignancies, including colorectal, breast, and lung cancers. Elevated CHKA activity promotes cell proliferation, survival, and metastasis by altering lipid signaling pathways and enhancing oncogenic Ras or AKT signaling.
CHKA-specific antibodies are essential tools for studying its expression, localization, and function in both normal and pathological contexts. They enable the detection of CHKA protein levels in tissues or cell lines via techniques like Western blot, immunohistochemistry, or immunofluorescence. Researchers also use these antibodies to explore CHKA's role in cancer progression and its potential as a therapeutic target. Inhibitors targeting CHKA enzymatic activity are under investigation, and antibodies aid in validating target engagement or assessing biomarker status in preclinical models.
The development of high-affinity, selective CHKA antibodies has advanced our understanding of its pathological mechanisms and spurred interest in CHKA as a diagnostic or prognostic marker in oncology.
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