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Mouse Decorin(DCN) ELISA kit

  • 中文名: Mouse Decorin(DCN) ELISA kit
货号: IPD-EL006554MO
Price: ¥2500
数量:
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产品详情

  • 产品描述:
    小鼠核心蛋白聚糖(DCN)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的DCN含量。DCN为一种靶点。它的背景可能与特定疾病的病理过程相关。研究机制方面,可能是其通过与相关分子相互作用,参与细胞信号传导等生理过程,进而影响疾病发展,未来或许可基于此开展疾病的诊断、治疗等方面的研究。试剂盒检测范围为78 pg/mL-5000 pg/mL,灵敏度为19.5 pg/mL。组织修复、纤维化疾病及肿瘤微环境研究;心血管疾病模型、皮肤组织再生、肝肺纤维化机制研究以及细胞外基质动态变化分析;探索DCN在病理生理过程中的调控作用本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
  • 别名:
    Dcn ELISA Kit; Decorin ELISA Kit; Bone proteoglycan II ELISA Kit; PG-S2 ELISA Kit; PG40 ELISA Kit
  • 缩写:
    DCN
  • Uniprot No.:
    P28654
  • 种属:
    Mus musculus (Mouse)
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    78 pg/mL-5000 pg/mL
  • 灵敏度:
    19.5 pg/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Signal Transduction
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of mouse DCN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    90


    Range %

    83-94


    1:2

    Average %

    107


    Range %

    97-112


    1:4

    Average %

    94


    Range %

    86-98


    1:8

    Average %

    88


    Range %

    81-92


  • 回收率:

    The recovery of mouse DCN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    94

    88-100


    EDTA plasma (n=4)

    88

    81-93


  • 标准曲线:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    pg/ml

    OD1

    OD2

    Average

    Corrected


    5000

    2.005

    1.911

    1.958

    1.808


    2500

    1.192

    1.142

    1.167

    1.017


    1250

    0.809

    0.789

    0.799

    0.649


    625

    0.490

    0.503

    0.497

    0.347


    312

    0.357

    0.366

    0.362

    0.212


    156

    0.280

    0.295

    0.288

    0.138


    78

    0.205

    0.214

    0.210

    0.060


    0

    0.148

    0.152

    0.150




  • 货期:
    3-5 working days

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