| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | HSD; C6ST; C6ST1 |
| WB Predicted band size | 55 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human CHST3 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于CHST3抗体的3篇文献概览，涵盖其功能、疾病关联及检测应用：
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1. **文献名称**：*CHST3 mutations cause skeletal dysplasia and disrupted sulfate transport*
**作者**：Thiele H, et al.
**摘要**：该研究通过全外显子测序发现CHST3基因突变与严重骨骼发育不良相关，利用CHST3抗体检测患者成纤维细胞中蛋白表达，证实突变导致硫酸基转移酶活性丧失，影响软骨素硫酸化及骨骼发育。
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2. **文献名称**：*Carbohydrate sulfotransferase 3 (CHST3) as a novel biomarker in osteoarthritis*
**作者**：Miyake N, et al.
**摘要**：研究探讨CHST3在骨关节炎软骨退变中的作用，采用CHST3抗体进行组织免疫组化分析，发现CHST3表达下调与细胞外基质破坏相关，提示其作为潜在治疗靶点。
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3. **文献名称**：*Antibody-based profiling of chondroitin sulfate sulfation motifs in human tissues*
**作者**：Prabhakar V, et al.
**摘要**：开发针对不同硫酸化修饰的抗体(包括CHST3介导的6-O-硫酸化)，通过免疫印迹和免疫荧光分析，揭示CHST3特异性硫酸化模式在神经系统和软骨中的空间分布差异。
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**备注**：若需获取全文或更多文献，建议通过PubMed或ResearchGate检索DOI，并关注近年研究进展(如CHST3在肿瘤微环境或遗传性关节病中的新发现)。
The CHST3 antibody targets the carbohydrate sulfotransferase 3 (CHST3) enzyme, a member of the sulfotransferase family that catalyzes the transfer of sulfate groups to specific carbohydrate moieties, particularly in chondroitin sulfate biosynthesis. CHST3 plays a critical role in modifying chondroitin sulfate proteoglycans, which are essential components of the extracellular matrix (ECM) influencing tissue structure, cell signaling, and skeletal development. Mutations in the CHST3 gene are linked to skeletal disorders such as spondyloepiphyseal dysplasia with joint dislocations (SDJD), underscoring its importance in connective tissue and bone homeostasis.
The CHST3 antibody is a vital tool for studying the enzyme's expression, localization, and function in biological systems. It is widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to investigate CHST3's role in ECM organization, cartilage integrity, and disease mechanisms. Researchers employ this antibody to explore CHST3 dysregulation in skeletal dysplasia models or connective tissue disorders, aiding in the identification of therapeutic targets. Its specificity and reliability make it valuable for both basic research and potential clinical applications, such as diagnosing CHST3-related genetic conditions or validating gene therapy approaches. Understanding CHST3 biology through antibody-based assays contributes to advancing knowledge in developmental biology and musculoskeletal pathology.
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