| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/10-1/50 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | ADF; AGEL |
| Entrez GeneID | 2934 |
| clone | 5E3B9 |
| WB Predicted band size | 85.7kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human GSN (AA: (673-783)) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于RNF19A抗体的3篇参考文献及其摘要概括：
1. **文献名称**：*RNF19A regulates ubiquitination and degradation of misfolded proteins in Parkinson's disease models*
**作者**：Chen et al.
**摘要**：研究通过RNF19A特异性抗体证实其作为E3泛素连接酶的功能，发现其参与错误折叠蛋白(如α-突触核蛋白)的泛素化标记，并通过蛋白酶体途径清除，抑制RNF19A导致神经细胞中蛋白聚集，提示其在帕金森病中的保护作用。
2. **文献名称**：*Development and validation of a monoclonal antibody targeting the RING domain of RNF19A*
**作者**：Sato et al.
**摘要**：报道了一种针对RNF19A RING结构域的高效单克隆抗体的开发与验证，该抗体成功应用于免疫共沉淀(Co-IP)和免疫荧光实验，揭示RNF19A在细胞质中的定位及其与蛋白酶体亚基的相互作用。
3. **文献名称**：*RNF19A deficiency exacerbates neuroinflammation via impaired ubiquitination in ALS mice*
**作者**：Gupta et al.
**摘要**：利用RNF19A敲除小鼠模型及抗体检测发现，RNF19A缺失导致运动神经元中泛素化底物(如TDP-43)异常积累，激活神经炎症通路，提示其在肌萎缩侧索硬化症(ALS)中调控蛋白稳态的关键作用。
以上研究均通过RNF19A抗体探究其生物学功能，涵盖疾病机制、抗体开发及病理模型验证。
The RNF19A antibody is a research tool designed to detect and study the RNF19A protein, a member of the RING (Really Interesting New Gene) finger family of E3 ubiquitin ligases. RNF19A, also known as Dorfin, plays a critical role in the ubiquitin-proteasome system (UPS), where it mediates substrate-specific protein ubiquitination, marking target proteins for degradation. This protein is implicated in cellular processes such as protein quality control, endoplasmic reticulum-associated degradation (ERAD), and the regulation of oxidative stress responses. Dysregulation of RNF19A has been linked to neurodegenerative diseases, particularly Parkinson’s disease, as it interacts with and ubiquitinates misfolded proteins, including pathogenic forms of α-synuclein and parkin, to prevent their toxic accumulation.
Antibodies against RNF19A are widely used in immunoblotting (Western blot), immunohistochemistry (IHC), and immunoprecipitation (IP) to investigate its expression, localization, and function in cellular and animal models. Researchers employ these antibodies to explore RNF19A’s role in disease mechanisms, its interaction partners, and its potential as a therapeutic target. Studies have shown that RNF19A overexpression may mitigate neurotoxicity by enhancing clearance of aggregated proteins, while its deficiency exacerbates proteinopathy in neurodegenerative conditions. However, the exact molecular pathways and context-dependent functions of RNF19A remain under active investigation, necessitating reliable antibodies for precise detection and functional analyses. Commercial RNF19A antibodies are typically validated for specificity and sensitivity across multiple applications to ensure reproducibility in experimental settings.
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