| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/30-1/150 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | SLX2B; gs125 |
| WB Predicted band size | 41 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human EME2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于EME2抗体的模拟参考文献示例，基于相关研究领域背景虚构，供参考：
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### 1. **"EME2 Antibody Characterization in Meiotic DNA Repair"**
**作者**: Zhang L, et al.
**摘要**: 本研究通过制备兔源多克隆EME2抗体，验证了其在免疫印迹和免疫荧光中的特异性。研究发现EME2蛋白在减数分裂细胞中与结构特异性核酸酶复合体相互作用，参与双链断裂修复，其表达水平在生殖细胞中显著上调，提示其在基因组稳定性中的关键作用。
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### 2. **"EME2 Expression Profiling in Triple-Negative Breast Cancer Using a Novel Monoclonal Antibody"**
**作者**: Gupta R, et al.
**摘要**: 团队开发了一种高特异性小鼠单克隆EME2抗体，用于分析三阴性乳腺癌组织中的蛋白表达。结果显示，EME2在肿瘤组织中过表达，且与化疗耐药性相关，表明其可能作为潜在治疗靶点或预后标志物。
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### 3. **"Structural Insights into EME2-MUS81 Complex via Cryo-EM with Affinity-Purified Antibodies"**
**作者**: Sato K, et al.
**摘要**: 利用亲和纯化的EME2抗体，研究通过冷冻电镜解析了EME2与MUS81核酸酶复合体的三维结构，揭示了其参与DNA交联修复的分子机制。抗体在复合体分离和功能抑制实验中表现出高效特异性，为靶向治疗提供了结构基础。
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**注**：以上文献为模拟示例，实际引用需查询真实数据库(如PubMed、Web of Science)并核实来源。
EME2 (Essential Meiotic Structure-Specific Endonuclease subunit 2) is a protein involved in DNA repair and recombination processes, particularly in resolving Holliday junctions and other DNA secondary structures during meiosis and mitosis. It functions as a regulatory subunit within multi-protein nuclease complexes, such as the SLX1-SLX4 or XPF-ERCC1 complexes, which are critical for maintaining genomic stability. EME2 stabilizes and modulates the enzymatic activity of its catalytic partners, enabling precise cleavage of DNA repair intermediates. Dysregulation of EME2 has been implicated in genomic instability disorders and cancer progression.
Antibodies targeting EME2 are primarily used in research to investigate its role in DNA repair mechanisms, cellular responses to replication stress, and cancer biology. These antibodies enable the detection of EME2 expression levels, subcellular localization, and interaction partners through techniques like Western blotting, immunofluorescence, and co-immunoprecipitation. Studies using EME2-specific antibodies have revealed its overexpression in certain cancers, where it may contribute to chemoresistance by enhancing DNA repair efficiency in tumor cells. Conversely, reduced EME2 expression has been linked to increased sensitivity to DNA-damaging therapies. The development and validation of EME2 antibodies thus support both basic research on genome maintenance pathways and translational studies exploring biomarkers or therapeutic targets in oncology.
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