| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/20-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | CTF; ECO1; EFO1; ESO1; A930014I12Rik |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human ESCO1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于ESCO1抗体的3篇参考文献，按您的要求整理：
1. **文献名称**：ESCO1 regulates sister chromatid cohesion by modulating the acetyltransferase activity of Esco1
**作者**：Hou F, Chu CW, Kong X, et al.
**摘要**：该研究揭示了ESCO1通过其乙酰转移酶活性调控姐妹染色单体黏连的分子机制，利用ESCO1抗体进行免疫共沉淀实验，证实ESCO1与黏连蛋白复合体相互作用，并对其功能进行验证。
2. **文献名称**：The acetyltransferase hEco1 interacts with and acetylates the cohesin subunit Smc3
**作者**：Unal E, Heidinger-Pauli JM, Kim W, et al.
**摘要**：文章通过ESCO1抗体的免疫荧光和Western blot分析，发现hEco1(ESCO1)通过乙酰化黏连蛋白亚基Smc3.在染色体分离和DNA修复中起关键作用，并揭示了其与癌症发生可能的关联。
3. **文献名称**：ESCO2 and ESCO1 have distinct functions in the regulation of cohesion and chromosome stability
**作者**：Whelan G, Kreidl E, Peters JM, et al.
**摘要**：研究比较ESCO1和ESCO2在染色体稳定性中的差异功能，通过ESCO1抗体敲低实验证明，ESCO1缺失导致姐妹染色单体黏连缺陷和基因组不稳定性，强调了其在细胞周期调控中的必要性。
若需具体DOI或发表年份，可进一步补充检索!
The ESCO1 (Establishment of Sister Chromatid Cohesion N-Acetyltransferase 1) antibody is a research tool used to study the ESCO1 protein, a key component of the cohesin complex critical for genomic stability. ESCO1. an acetyltransferase, facilitates sister chromatid cohesion during DNA replication by acetylating the cohesin subunit SMC3. ensuring proper chromosome segregation in mitosis and meiosis. Dysregulation of ESCO1 is linked to developmental disorders, cancer, and chromosomal instability syndromes like Roberts syndrome.
ESCO1 antibodies are primarily employed in techniques such as Western blotting, immunofluorescence, and chromatin immunoprecipitation (ChIP) to detect protein expression, localization, and interactions. They help investigate ESCO1's role in cell cycle regulation, DNA repair, and its crosstalk with other epigenetic modifiers. Recent studies also explore ESCO1's involvement in transcriptional regulation beyond cohesion, highlighting its broader impact on chromatin dynamics.
Commercial ESCO1 antibodies are typically validated in specific applications and model systems, with considerations for species reactivity (e.g., human, mouse). Researchers rely on these antibodies to dissect molecular mechanisms underlying cohesion defects and to identify therapeutic targets in diseases associated with cohesinopathies or aneuploidy. However, variability in antibody specificity necessitates careful validation to avoid off-target effects in experimental settings.
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