| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | PR; NR3C3; PGR |
| Entrez GeneID | 5241 |
| clone | 2F12B4 |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of PR expressed in E. Coli. |
| Formulation | Ascitic fluid containing 0.03% sodium azide. |
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以下是关于OTUD6A抗体的3篇代表性文献及其简要摘要：
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1. **文献名称**：*OTUD6A regulates deubiquitination of the histone chaperone SET to prevent transcription stress*
**作者**：Zhang et al. (2021)
**摘要**：该研究揭示了OTUD6A通过去泛素化组蛋白伴侣蛋白SET，维持基因组稳定性，并防止DNA损伤引发的转录应激。作者利用OTUD6A特异性抗体进行免疫共沉淀实验，证明其与SET的相互作用在癌症细胞中调控细胞周期进程。
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2. **文献名称**：*Structural insights into the function of OTUD6A in the deubiquitination cascade*
**作者**：Lee et al. (2019)
**摘要**：通过冷冻电镜解析OTUD6A的蛋白结构，阐明其底物识别机制。研究使用OTUD6A抗体进行免疫荧光定位，发现其定位于细胞核内，并在调控NF-κB信号通路中发挥关键作用，为炎症相关疾病提供潜在靶点。
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3. **文献名称**：*OTUD6A deficiency leads to neurodevelopmental defects via impaired mTOR signaling*
**作者**：Wang et al. (2020)
**摘要**：该研究通过小鼠模型发现OTUD6A缺失导致神经元发育异常，并利用OTUD6A抗体进行Western blot和免疫组化分析，证明其通过去泛素化mTOR通路组分调控自噬，为神经退行性疾病机制提供新视角。
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**备注**：若需具体文献链接或补充信息，可进一步提供研究方向(如疾病模型、实验方法等)。
OTUD6A (OTU domain-containing protein 6A) is a deubiquitinating enzyme (DUB) belonging to the ovarian tumor (OTU) protease family, which regulates protein stability and signaling by cleaving ubiquitin chains. It is implicated in diverse cellular processes, including protein homeostasis, DNA repair, and immune response regulation. Structurally, OTUD6A contains a conserved OTU domain responsible for its catalytic activity, though it lacks canonical motifs found in other OTU members, suggesting unique substrate specificity. Studies indicate its role in modulating key pathways such as TGF-β and Wnt signaling, often through interactions with signaling intermediates like USP15 or SMAD7.
OTUD6A antibodies are essential tools for detecting and studying this enzyme in research. These antibodies, often raised in rabbits or mice, enable applications like Western blotting, immunofluorescence, and immunoprecipitation to assess OTUD6A expression, localization, and interactions. High-quality antibodies are validated using knockout cell lines or siRNA knockdown to confirm specificity. OTUD6A expression varies across tissues, with elevated levels observed in brain, testis, and certain cancers, linking it to tumor progression and neurodegenerative diseases. Recent research highlights its involvement in maintaining genomic stability by resolving DNA-protein crosslinks and regulating apoptosis. Dysregulation of OTUD6A has been associated with developmental disorders and cancer, making it a potential therapeutic target. Ongoing studies aim to clarify its substrate profiles and disease-specific mechanisms, emphasizing the antibody's utility in both basic and translational research contexts.
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