| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/30-1/150 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| WB Predicted band size | 34 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human EN2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇与EN2抗体相关的文献示例(内容基于公开研究整理，部分信息可能简化)：
1. **文献名称**：*Engrailed-2 (EN2) as a urinary biomarker for prostate cancer detection*
**作者**：Morgan R, et al.
**摘要**：该研究提出EN2蛋白可作为前列腺癌的无创诊断标志物，通过ELISA法检测尿液中的EN2水平。研究中使用特异性EN2抗体进行定量分析，结果显示EN2在前列腺癌患者尿液中显著高表达，具有较高的敏感性和特异性。
2. **文献名称**：*The role of Engrailed-2 in tumorigenesis and its interaction with Wnt/β-catenin signaling*
**作者**：Zhang Y, et al.
**摘要**：本文探讨EN2在肿瘤发生中的作用，发现EN2通过激活Wnt/β-catenin通路促进细胞增殖和侵袭。实验中采用EN2抗体进行免疫组化和Western blot分析，证实其在乳腺癌和结肠癌组织中过表达，并与患者预后不良相关。
3. **文献名称**：*Development of a monoclonal antibody against EN2 for therapeutic targeting in neuroblastoma*
**作者**：Lee S, et al.
**摘要**：研究团队开发了一种靶向EN2的单克隆抗体，验证其在神经母细胞瘤中的治疗潜力。体内外实验显示，该抗体可特异性结合EN2阳性肿瘤细胞，抑制生长并诱导凋亡，为EN2靶向治疗提供新策略。
(注：以上文献为示例，实际引用需核对真实出版物信息。)
EN2 (Engrailed-2) is a homeobox-containing transcription factor encoded by the *EN2* gene, primarily known for its role in embryonic development, particularly in the patterning of the midbrain-hindbrain region and cerebellar development. Initially studied in neurodevelopment, EN2 has gained attention in cancer research due to its aberrant expression in malignancies such as prostate, breast, and lung cancers. In tumors, EN2 acts as an oncoprotein, promoting cell proliferation, invasion, and metastasis while suppressing apoptosis. Its expression in cancer cells correlates with advanced disease stages and poor prognosis, suggesting potential utility as a diagnostic or prognostic biomarker.
EN2 antibodies are tools developed to detect and quantify EN2 protein levels in research and clinical contexts. Polyclonal and monoclonal EN2 antibodies are used in techniques like immunohistochemistry, Western blotting, and ELISA to study EN2 localization, expression patterns, and interactions. In prostate cancer, EN2 antibodies have been pivotal in identifying secretory EN2 in urine, a non-invasive diagnostic marker. Additionally, EN2-targeting antibodies are being explored for therapeutic applications, including antibody-drug conjugates or immune-based therapies. Beyond oncology, EN2 antibodies contribute to neuroscience research, elucidating its role in neurodegenerative disorders like Alzheimer’s disease. Despite progress, challenges remain in standardizing antibody specificity and optimizing clinical translation.
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