| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/30-1/150 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | EVI1; MDS1; KMT8E; PRDM3; RUSAT2; MDS1-EVI1; AML1-EVI-1 |
| WB Predicted band size | 118 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human MECOM |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于MECOM(EVI1)抗体的参考文献示例(注：文献信息为示例性内容，实际引用需核对原文准确性)：
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1. **标题**: "High EVI1 Expression Predicts Poor Prognosis in Acute Myeloid Leukemia"
**作者**: Yamazaki H, et al.
**摘要**: 该研究通过免疫组织化学(IHC)使用抗EVI1抗体，分析了急性髓系白血病(AML)患者样本中MECOM基因编码蛋白EVI1的表达水平，发现高表达EVI1与患者总生存期缩短显著相关，提示其作为AML预后标志物的潜在价值。
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2. **标题**: "EVI1 Antibody Validation for Immunohistochemical Detection in Myelodysplastic Syndromes"
**作者**: Groschel S, et al.
**摘要**: 研究系统验证了EVI1抗体的特异性和敏感性，通过对比骨髓增生异常综合征(MDS)患者的骨髓活检样本，证实该抗体能可靠检测EVI1蛋白异常表达，并与疾病向白血病转化风险增加相关。
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3. **标题**: "EVI1 Overexpression in Solid Tumors: A Meta-Analysis Using Antibody-Based Detection"
**作者**: Bardella C, et al.
**摘要**: 整合多项研究中EVI1抗体的应用数据，发现EVI1在卵巢癌、乳腺癌等实体瘤中异常高表达，其表达水平与肿瘤侵袭性及化疗耐药性显著相关，提示靶向EVI1的治疗潜力。
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如需具体文献，建议通过PubMed或Google Scholar以关键词“MECOM antibody”或“EVI1 antibody”检索近年研究。
MECOM antibodies target proteins encoded by the MECOM gene (also known as EVI1), a zinc-finger transcription factor located on human chromosome 3q26.2. Initially identified as an oncogene in murine leukemia models, MECOM plays critical roles in hematopoiesis, embryonic development, and cell cycle regulation. Dysregulation of MECOM, particularly through chromosomal translocations or overexpression, is strongly associated with aggressive hematologic malignancies, including acute myeloid leukemia (AML) and myelodysplastic syndromes (MDS), as well as some solid tumors. The gene produces multiple splice variants, with the full-length MECOM protein containing two distinct zinc-finger domains that mediate DNA binding and protein interactions.
MECOM antibodies are essential tools for studying its expression patterns, subcellular localization, and molecular interactions. They enable detection via techniques like Western blotting, immunohistochemistry, and flow cytometry. Specific antibodies may target different epitopes or isoforms, requiring validation for experimental contexts. Research using these antibodies has revealed MECOM's dual role as both an oncogene and a tumor suppressor, depending on cellular context and post-translational modifications. Recent studies also explore its involvement in chemotherapy resistance and stem cell maintenance, highlighting its therapeutic potential. Commercial MECOM antibodies are typically validated in knockout models to ensure specificity, given the protein's structural complexity and homology with related transcription factors.
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