| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | Fbx32; MAFbx |
| WB Predicted band size | 42 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human FBXO32 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇涉及FBXO32(Atrogin-1/MAFbx)抗体的代表性文献摘要：
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1. **文献名称**: Identification of ubiquitin ligases required for skeletal muscle atrophy
**作者**: Bodine SC, et al.
**摘要**: 该研究首次报道FBXO32(Atrogin-1)作为肌肉特异性E3泛素连接酶，在去神经或糖皮质激素诱导的肌肉萎缩模型中表达显著上调。通过Western blot和免疫组化使用FBXO32抗体，证实其通过促进肌细胞蛋白降解参与萎缩过程。
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2. **文献名称**: Multiple types of skeletal muscle atrophy involve a common program of ubiquitin ligase induction
**作者**: Lecker SH, et al.
**摘要**: 研究比较了癌症恶病质、糖尿病和肾衰竭等多种肌肉萎缩模型，发现FBXO32抗体检测显示其表达水平普遍升高。提示FBXO32是多种病理条件下肌肉降解的共同调控因子。
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3. **文献名称**: FBXO32 promotes breast cancer cell metastasis via regulation of glycolytic metabolism
**作者**: Cao Y, et al.
**摘要**: 该研究利用FBXO32抗体进行免疫沉淀和质谱分析，发现FBXO32通过调控糖酵解关键酶稳定性促进乳腺癌转移，揭示了其在肿瘤代谢中的新功能。
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4. **文献名称**: Atrogin-1/MAFbx regulates Alzheimer’s disease-associated synaptic plasticity deficits
**作者**: Paul A, et al.
**摘要**: 在阿尔茨海默病小鼠模型中，FBXO32抗体染色显示其海马区表达异常升高，通过泛素化降解突触蛋白PSD-95.导致突触功能障碍。抑制FBXO32可改善认知缺陷。
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注：上述文献为领域内典型研究方向示例，实际引用时建议通过PubMed/Google Scholar核实具体文献信息。
The FBXO32 antibody is a research tool targeting FBXO32 (F-box protein 32), also known as Atrogin-1 or MAFbx, a key component of the ubiquitin-proteasome system. FBXO32 functions as a substrate-recognition subunit within the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex, directing specific proteins for proteasomal degradation. It plays a critical role in muscle atrophy by ubiquitinating proteins like MyoD and eIF3-f, thereby regulating skeletal muscle mass under conditions such as starvation, disuse, or disease.
FBXO32 is implicated in multiple pathways, including the AKT/mTOR signaling axis, and its expression is upregulated in catabolic states. Beyond muscle biology, it has been linked to cancer progression, neurodegenerative diseases, and cardiac remodeling. The FBXO32 antibody is widely used in Western blotting, immunohistochemistry, and immunofluorescence to study protein expression, localization, and degradation mechanisms in these contexts.
Researchers utilize this antibody to explore FBXO32's role in cellular homeostasis, disease pathogenesis, and therapeutic targeting. Validation methods (e.g., knockout controls) are essential due to potential cross-reactivity with related F-box proteins. Its applications span basic research, drug development, and biomarker studies, particularly in disorders involving protein turnover dysregulation.
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