| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MED4 |
| Uniprot No | Q9NPJ6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-270aa |
| 氨基酸序列 | MAASSSGEKEKERLGGGLGVAGGNSTRERLLSALEDLEVLSRELIEMLAISRNQKLLQAGEENQVLELLIHRDGEFQELMKLALNQGKIHHEMQVLEKEVEKRDSDIQQLQKQLKEAEQILATAVYQAKEKLKSIEKARKGAISSEEIIKYAHRISASNAVCAPLTWVPGDPRRPYPTDLEMRSGLLGQMNNPSTNGVNGHLPGDALAAGRLPDVLAPQYPWQSNDMSMNMLPPNHSSDFLLEPPGHNKENEDDVEIMSTDSSSSSSESD |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MED4重组蛋白的3篇参考文献的简要总结：
1. **文献名称**: "Recombinant Expression and Functional Characterization of Mediator Complex Subunit 4 in Arabidopsis"
**作者**: Smith J, et al.
**摘要**: 该研究成功在大肠杆菌中表达了拟南芥MED4重组蛋白，并验证其在中介体复合物中的结构作用。实验表明，重组MED4能够恢复突变体植株的转录缺陷，证实其在RNA聚合酶II调控中的关键功能。
2. **文献名称**: "Structural Analysis of MED4 in the Human Mediator Complex"
**作者**: Lee H, et al.
**摘要**: 通过昆虫细胞系统表达人源MED4重组蛋白，结合冷冻电镜解析其三维结构。研究发现MED4通过特定结构域与MED9和MED31亚基互作，揭示了中介体复合物组装的新机制。
3. **文献名称**: "MED4 Recombinant Protein Enhances Transcriptional Activation in Yeast Models"
**作者**: Garcia R, et al.
**摘要**: 在酿酒酵母中异源表达MED4重组蛋白，发现其显著增强特定基因启动子的转录活性。研究为MED4在真核生物转录调控中的进化保守性提供了证据。
注：以上文献信息为示例性内容，实际引用时需根据具体研究核实原文。
**Background of MED4 Recombinant Protein**
The MED4 protein, also known as Mediator Complex Subunit 4. is a critical component of the Mediator complex—a multi-subunit assembly that regulates transcription in eukaryotic cells. This evolutionarily conserved complex acts as a molecular bridge, facilitating communication between transcription factors, RNA polymerase II, and chromatin-modifying enzymes to fine-tune gene expression. MED4 is part of the Mediator’s core module, contributing to structural stability and mediating interactions essential for transcriptional initiation and elongation.
MED4 plays a pivotal role in cellular processes such as cell cycle regulation, differentiation, and stress responses. Dysregulation of MED4 has been implicated in diseases, including cancers, where aberrant transcriptional programs drive tumor progression. For example, altered MED4 expression correlates with poor prognosis in certain malignancies, highlighting its potential as a therapeutic target.
Recombinant MED4 protein is produced via heterologous expression systems (e.g., *E. coli* or mammalian cells) to study its biochemical properties, interactions, and functional mechanisms. Its purified form enables *in vitro* assays, structural studies (e.g., crystallography or cryo-EM), and drug discovery efforts aimed at modulating Mediator complex activity. Researchers also utilize MED4 recombinant proteins to investigate its role in developmental disorders or metabolic diseases linked to transcriptional dysregulation.
Recent studies emphasize MED4’s involvement in tissue-specific gene regulation, particularly in stem cells and immune responses, underscoring its broader biological significance. Advances in recombinant protein technology continue to enhance our understanding of MED4’s molecular functions, offering insights into novel therapeutic strategies for transcription-related pathologies.
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