| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MED21 |
| Uniprot No | Q13503 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-144aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMADRLTQ LQDAVNSLAD QFCNAIGVLQ QCGPPASFNN IQTAINKDQP ANPTEEYAQL FAALIARTAK DIDVLIDSLP SEESTAALQA ASLYKLEEEN HEAATCLEDV VYRGDMLLEK IQSALADIAQ SQLKTRSGTH SQSLPDS |
| 预测分子量 | 18 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MED21重组蛋白的3篇参考文献示例：
1. **《MED21 regulates the transcription cycle by interacting with RNA polymerase II》**
- **作者**：Smith A, et al.
- **摘要**：研究通过重组表达MED21蛋白，揭示了其与RNA聚合酶II的直接相互作用，证明MED21在中介体复合物中调控转录起始和延伸的关键作用。
2. **《Structural insights into the Mediator complex: Role of MED21 in subunit assembly》**
- **作者**：Chen L, et al.
- **摘要**：利用重组MED21蛋白进行晶体结构分析，阐明了MED21在中介体复合物亚基组装中的结构基础，并发现其C端结构域对复合物稳定性至关重要。
3. **《Functional characterization of MED21 in fungal stress response pathways》**
- **作者**：Wang X, et al.
- **摘要**：通过酵母重组MED21蛋白的遗传和生化分析，发现MED21参与胁迫响应基因的转录调控，缺失导致细胞对氧化应激的敏感性增加。
(注：上述文献为示例，实际文献需通过学术数据库检索确认。)
MED21. a subunit of the mediator complex, plays a critical role in transcriptional regulation across eukaryotes. The mediator complex acts as a molecular bridge, facilitating communication between transcription factors and RNA polymerase II to regulate gene expression. MED21 (also known as Srb7) is part of the conserved core module of the mediator, contributing to structural stability and functional interactions. Studies have linked MED21 to diverse cellular processes, including cell cycle progression, stress responses, and metabolic regulation.
Recombinant MED21 protein is engineered for experimental studies to dissect its molecular mechanisms. Produced via heterologous expression systems (e.g., E. coli or mammalian cells), the recombinant protein retains key functional domains, enabling researchers to analyze its interactions with other mediator subunits, DNA-binding transcription factors, or RNA polymerase II. Purification methods often involve affinity tags (e.g., His-tag or GST-tag) for efficient isolation.
Research using recombinant MED21 has shed light on its role in diseases. Dysregulation of MED21 is associated with cancers, neurodegenerative disorders, and viral infections. For example, it interacts with oncogenic transcription factors, influencing tumor progression. Additionally, MED21 participates in HIV replication by modulating viral gene transcription. These findings highlight its potential as a therapeutic target.
The protein’s structural and functional conservation across species makes it valuable for comparative studies. However, challenges remain in understanding its post-translational modifications and context-dependent roles. Recombinant MED21 tools continue to advance mechanistic studies, drug screening, and structural biology efforts aimed at unraveling the mediator complex’s complexity in health and disease.
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