| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MEIS3 |
| Uniprot No | Q99687 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-358aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMARRYDE LPHYPGIVDG PAALASFPET VPAVPGPYGP HRPPQPLPPG LDSDGLKREK DEIYGHPLFP LLALVFEKCE LATCSPRDGA GAGLGTPPGG DVCSSDSFNE DIAAFAKQVR SERPLFSSNP ELDNLMIQAI QVLRFHLLEL EKGKMPIDLV IEDRDGGCRE DFEDYPASCP SLPDQNNMWI RDHEDSGSVH LGTPGPSSGG LASQSGDNSS DQGDGLDTSV ASPSSGGEDE DLDQERRRNK KRGIFPKVAT NIMRAWLFQH LSHPYPSEEQ KKQLAQDTGL TILQVNNWFI NARRRIVQPM IDQSNRTGQG AAFSPEGQPI GGYTETQPHV AVRPPGSVGM SLNLEGEWHY L |
| 预测分子量 | 41 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MEIS3重组蛋白的3篇参考文献示例，涵盖其功能研究与制备应用：
1. **"Recombinant MEIS3 Protein Regulates Neural Crest Cell Migration in Xenopus"**
*作者：Smith A, et al.*
摘要：通过表达纯化MEIS3重组蛋白，研究发现其通过调控Wnt信号通路影响非洲爪蟾神经嵴细胞的迁移，揭示了MEIS3在胚胎发育中的关键作用。
2. **"Structural Characterization of MEIS3 Homeodomain Using X-ray Crystallography"**
*作者：Chen L, et al.*
摘要：首次解析了MEIS3同源结构域的晶体结构，揭示其DNA结合位点的特异性，为研究MEIS家族蛋白的分子机制提供了结构基础。
3. **"MEIS3 Recombinant Protein Enhances Hematopoietic Stem Cell Differentiation"**
*作者：Kimura R, et al.*
摘要：利用大肠杆菌系统表达MEIS3重组蛋白，证明其与HOXA9协同促进小鼠造血干细胞的体外分化，提示其在血液疾病治疗中的潜在应用。
(注：以上文献为示例，实际研究中建议通过学术数据库验证最新成果。)
**Background of MEIS3 Recombinant Protein**
MEIS3 (Myeloid Ecotropic Viral Integration Site 3) belongs to the MEIS family of homeobox transcription factors, which play pivotal roles in embryonic development, cellular differentiation, and tissue patterning. These proteins are characterized by a conserved homeodomain that facilitates DNA binding and interaction with co-factors like PBX (Pre-B-cell leukemia homeobox) and HOX (Homeobox) proteins, forming complexes to regulate gene expression. MEIS3. specifically, is implicated in neurodevelopment, hematopoiesis, and organogenesis, with studies highlighting its involvement in neural crest cell migration, neuronal subtype specification, and limb development.
The recombinant MEIS3 protein is engineered using molecular cloning techniques, often expressed in bacterial or mammalian systems to ensure proper folding and post-translational modifications. This engineered protein retains the functional domains of native MEIS3. enabling its use in *in vitro* and *in vivo* studies to dissect molecular mechanisms. Researchers employ MEIS3 recombinant protein to investigate its DNA-binding properties, transcriptional regulatory activity, and interactions with signaling pathways such as Wnt or TGF-β.
Dysregulation of MEIS3 is linked to developmental disorders and cancers, including leukemia and neuroblastoma, underscoring its biomedical relevance. The availability of recombinant MEIS3 aids in drug discovery, structural studies, and functional assays, providing insights into its role in health and disease. Its application spans developmental biology, cancer research, and regenerative medicine, making it a critical tool for understanding transcriptional regulation and cellular fate decisions.
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