| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/20-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | apolipoprotein A-IV |
| Entrez GeneID | 337 |
| clone | 1D4C11 |
| WB Predicted band size | 45kDa |
| Host/Isotype | Mouse IgG2a |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of APOA4 (aa21-396) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇与MTIF2抗体相关的文献概览：
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1. **文献名称**：*Mitochondrial translation initiation factor 2 interacts with a conserved stem-loop structure in mitochondrial mRNAs*
**作者**：Bhargava, K., & Spremulli, L.L.
**摘要**：该研究通过免疫共沉淀和Western blot技术，利用MTIF2特异性抗体验证了MTIF2蛋白与线粒体mRNA特定茎环结构的直接结合，揭示了其在翻译起始中的调控作用。
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2. **文献名称**：*Defective mitochondrial translation caused by MTIF2 mutations leads to early-onset encephalopathy*
**作者**：Gardeitchik, T., et al.
**摘要**：文章通过患者样本的免疫组化分析(使用MTIF2抗体)，发现MTIF2基因突变导致线粒体翻译缺陷，进而引发神经退行性疾病，为相关疾病的诊断提供了分子依据。
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3. **文献名称**：*MTIF2 depletion disrupts mitochondrial ribosome assembly and respiratory chain function*
**作者**：Lee, S.G., et al.
**摘要**：利用MTIF2抗体敲低细胞模型，研究发现MTIF2缺失会破坏线粒体核糖体组装，降低呼吸链复合体活性，强调了其在维持线粒体功能中的必要性。
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**备注**：若需获取具体文献，建议通过PubMed或期刊数据库检索标题及作者，结合研究领域(如线粒体疾病、翻译机制)可进一步扩展相关文献。
The mitochondrial translation initiation factor 2 (MTIF2), also known as IF2mt, is a critical protein involved in initiating protein synthesis within mitochondria. It facilitates the assembly of the mitochondrial ribosome by binding to the small subunit (28S) and recruiting the initiator tRNA (fMet-tRNA) to start translation. MTIF2 is essential for the synthesis of mitochondrial DNA-encoded proteins, particularly components of the electron transport chain (ETC) complexes crucial for oxidative phosphorylation and cellular energy production. Structurally, it contains GTP-binding domains and interacts with mitochondrial ribosomes in a GTP-dependent manner.
Antibodies targeting MTIF2 are valuable tools for studying mitochondrial translation mechanisms, mitochondrial dysfunction, and associated diseases. Dysregulation of MTIF2 has been linked to disorders such as mitochondrial encephalopathies, cardiomyopathies, and cancer, where impaired energy metabolism plays a role. Researchers use MTIF2 antibodies in techniques like Western blotting, immunofluorescence, and immunoprecipitation to assess protein expression, localization, and interactions in cellular or tissue samples. These studies help elucidate how mitochondrial translation defects contribute to pathological conditions, offering potential insights for therapeutic strategies targeting mitochondrial protein synthesis. Commercial MTIF2 antibodies are typically validated for specificity using knockout controls or siRNA-based knockdowns.
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