Rabbit Polyclonal HRAS Antibody

The HRAS antibody is a crucial tool in molecular biology and cancer research, targeting the H-Ras protein encoded by the HRAS proto-oncogene. H-Ras belongs to the RAS family of small GTPases, which regulate key cellular processes like proliferation, differentiation, and apoptosis by cycling between active GTP-bound and inactive GDP-bound states. Mutations in HRAS, particularly at codons 12. 13. or 61. result in constitutive activation of the protein, driving uncontrolled cell growth and contributing to various cancers, including bladder, thyroid, and squamous cell carcinomas. HRAS antibodies are widely used to detect H-Ras expression levels, localization, and activation status in tissues or cell lines, aiding in the study of oncogenic signaling pathways such as MAPK/ERK and PI3K/AKT. These antibodies are essential for techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF), helping to identify HRAS-driven malignancies or validate experimental models. Researchers also utilize them to explore therapeutic resistance mechanisms or evaluate targeted therapies in preclinical studies. Specificity is critical, as cross-reactivity with other RAS isoforms (KRAS, NRAS) can occur; thus, rigorous validation using knockout controls or mutation-specific assays is recommended. Both monoclonal and polyclonal HRAS antibodies are available, with selection depending on the required sensitivity and application context.