| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | HMD; IFAP2; SREBP1; bHLHd1 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human SREBF1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于SREBF1抗体的3篇参考文献示例(文献信息为模拟，仅供参考)：
1. **文献名称**：*SREBP-1 regulates lipid metabolism in hepatic steatosis via antibody-based detection*
**作者**：Brown MS, Goldstein JL
**摘要**：本研究利用SREBF1特异性抗体(克隆2A4)通过Western blot和免疫组化分析高脂饮食小鼠肝脏中SREBF1蛋白的激活形式(成熟核片段)。发现SREBF1在脂肪肝中显著上调，且与脂肪酸合成相关基因表达正相关。
2. **文献名称**：*Antibody validation for SREBF1 isoforms in human adipocytes*
**作者**：Horton JD, et al.
**摘要**：作者通过siRNA敲低和免疫荧光技术验证了商业抗体(如Abcam ab3259)对人前脂肪细胞中SREBF1两种异构体(SREBP-1a和SREBP-1c)的特异性，并证明其在细胞核内的定位受胰岛素调控。
3. **文献名称**：*SREBP-1 antibody application in neurodegenerative disease models*
**作者**：Saito R, et al.
**摘要**：研究使用SREBF1抗体(CST #9874)检测阿尔茨海默病模型小鼠脑组织中SREBF1的表达变化，发现其与神经元脂质沉积和炎症信号通路激活相关，提示SREBF1可能参与神经退行性病变的脂代谢异常。
注：以上文献信息为示例性质，实际引用需根据真实文献调整。建议通过PubMed或Google Scholar以“SREBF1 antibody”或“SREBP-1 detection”为关键词检索最新研究。
The Sterol Regulatory Element-Binding Transcription Factor 1 (SREBF1), also known as SREBP-1. is a key regulator of lipid metabolism. It belongs to the SREBP family of transcription factors that control cellular cholesterol and fatty acid homeostasis. SREBF1 exists in two isoforms (SREBP-1a and SREBP-1c) generated by alternative splicing, with SREBP-1c primarily driving fatty acid synthesis by activating genes like *FASN* (fatty acid synthase) and *ACC* (acetyl-CoA carboxylase). It is synthesized as an endoplasmic reticulum (ER)-bound precursor and undergoes proteolytic cleavage to release its active nuclear form when cellular sterol levels are low. Dysregulation of SREBF1 is linked to metabolic disorders (e.g., obesity, diabetes, NAFLD) and cancers reliant on lipogenesis.
SREBF1 antibodies are essential tools for detecting and studying this protein in research. These antibodies, often monoclonal or polyclonal, are validated for applications such as Western blotting, immunofluorescence, chromatin immunoprecipitation (ChIP), and immunohistochemistry. Specificity is critical, as cross-reactivity with other SREBP family members (e.g., SREBF2) must be ruled out. High-quality SREBF1 antibodies enable investigations into its expression patterns, post-translational modifications, and interactions in metabolic or oncogenic pathways. Researchers rely on these reagents to explore therapeutic targets for diseases associated with lipid dysregulation, emphasizing the antibody's role in advancing mechanistic and translational studies.
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