| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | FBX1; FBXO1 |
| WB Predicted band size | 88 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human CCNF |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于CCNF(Cyclin F)抗体的模拟参考文献示例，供参考(建议通过学术数据库进一步验证信息的准确性)：
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1. **文献名称**：*Cyclin F regulates the ubiquitination and degradation of substrates critical for G2/M progression*
**作者**：D’Angiolella, V., et al.
**摘要**：该研究揭示了Cyclin F通过SCF泛素连接酶复合体调控细胞周期G2/M期关键蛋白的降解机制。研究使用特异性CCNF抗体验证其与底物蛋白的相互作用，并证实其在维持基因组稳定性中的作用。
2. **文献名称**：*Aberrant Cyclin F expression in breast cancer correlates with tumor progression*
**作者**：Smith, J., et al.
**摘要**：通过免疫组化(IHC)和Western blot分析，研究发现CCNF在乳腺癌组织中表达异常升高，且与患者预后不良相关。研究强调CCNF抗体在临床样本检测中的应用价值。
3. **文献名称**：*CCNF mutations disrupt protein degradation pathways in ALS pathogenesis*
**作者**：Gitler, A. D., et al.
**摘要**：文章发现肌萎缩侧索硬化症(ALS)患者中CCNF基因突变导致异常蛋白聚集。通过抗体验证，突变的CCNF蛋白稳定性增加，提示其与神经退行性病变的关联。
4. **文献名称**：*Development and validation of a high-specificity Cyclin F antibody for functional proteomics*
**作者**：Lee, C., et al.
**摘要**：该文献描述了兔源单克隆CCNF抗体的制备过程，通过免疫沉淀(IP)和质谱分析验证其特异性，为研究CCNF相互作用组提供可靠工具。
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**注意**：上述文献为模拟示例，实际研究中请通过PubMed、Google Scholar等平台检索真实文献(关键词：Cyclin F antibody、CCNF function、ubiquitination等)。
CCNF (Cyclin F) antibodies are essential tools in studying the regulatory mechanisms of the cell cycle, particularly during the G2/M phase transition. Cyclin F, encoded by the *CCNF* gene, is a member of the F-box protein family and a component of the Skp1-Cul1-F-box (SCF) ubiquitin ligase complex. Unlike classical cyclins, Cyclin F does not activate cyclin-dependent kinases (CDKs) but instead controls cell cycle progression by targeting specific substrates for ubiquitination and proteasomal degradation. Key substrates include proteins involved in DNA replication, centrosome duplication, and mitotic progression, such as CDC6. CP110. and RRM2. Dysregulation of Cyclin F has been linked to genomic instability, cancer progression, and neurodegenerative disorders like amyotrophic lateral sclerosis (ALS).
CCNF antibodies are widely used in research to detect Cyclin F expression levels, localization, and interactions via techniques like Western blotting, immunofluorescence, and immunoprecipitation. They help elucidate Cyclin F's role in maintaining genome integrity, its temporal regulation during the cell cycle, and its pathological implications. Recent studies also explore its potential as a therapeutic target, particularly in cancers with Cyclin F overexpression or mutations. These antibodies are critical for advancing understanding of cell cycle checkpoints and developing strategies to address Cyclin F-related diseases.
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