| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | CP24; HCAI; CYP24; HCINF1; P450-CC24 |
| WB Predicted band size | 59 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Rat |
| Immunogen | Synthetic peptide of human CYP24A1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于CYP24A1抗体的3篇参考文献示例(内容为虚构示例，仅供参考)：
1. **文献名称**: "CYP24A1 Antibody Localization and Expression in Renal Tissues"
**作者**: Anderson et al.
**摘要**: 研究利用特异性CYP24A1抗体，通过免疫组化技术揭示了其在肾脏近端小管中的高表达，证实了其在维生素D代谢中的关键作用，并探讨了慢性肾病中CYP24A1表达下调的机制。
2. **文献名称**: "Development of a Monoclonal Antibody for Human CYP24A1: Applications in Cancer Research"
**作者**: Sakaki et al.
**摘要**: 本文报道了一种高特异性单克隆抗体的开发，用于检测CYP24A1在结直肠癌组织中的过表达，揭示了其与维生素D活性代谢物降解及肿瘤进展的潜在关联。
3. **文献名称**: "CYP24A1 Genetic Mutations and Antibody-Based Diagnostic Approaches"
**作者**: Nesterova et al.
**摘要**: 研究通过Western blot和免疫荧光技术，利用CYP24A1抗体分析遗传性维生素D代谢异常患者的突变蛋白表达，为临床诊断提供了分子生物学依据。
(注：以上文献及作者为示例，实际引用需根据真实数据库检索。)
The CYP24A1 antibody targets the enzyme cytochrome P450 family 24 subfamily A member 1 (CYP24A1), a key regulator of vitamin D metabolism. This mitochondrial enzyme catalyzes the hydroxylation of active 1.25-dihydroxyvitamin D3 (calcitriol) and 25-hydroxyvitamin D3 into inactive metabolites, maintaining vitamin D homeostasis and preventing hypercalcemia. Dysregulation of CYP24A1 is linked to disorders like idiopathic infantile hypercalcemia (IIH) and chronic kidney disease, as well as cancers where overexpression may promote tumor progression by degrading anti-proliferative calcitriol.
CYP24A1 antibodies are widely used in research to study tissue-specific expression patterns, enzyme activity, and pathological mechanisms. They enable detection of CYP24A1 protein levels via techniques like immunohistochemistry, Western blotting, and ELISA. Commercially available antibodies are typically raised against specific epitopes of human CYP24A1. validated for specificity in various models. Clinically, these antibodies may aid in diagnosing genetic CYP24A1 deficiencies or assessing vitamin D status in metabolic diseases. Recent studies also explore their potential in cancer prognosis, as elevated CYP24A1 expression correlates with reduced calcitriol efficacy in malignancies. However, standardization of antibody validation remains critical to ensure accuracy across experimental and diagnostic applications.
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