| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/500-1/5000 | Human,Mouse,Rat |
| Aliases | STAT5 |
| WB Predicted band size | 90 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human STAT5B |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3条关于STAT5B抗体的参考文献(基于模拟生成，非真实文献)：
1. **文献名称**: "STAT5B signaling in cancer progression and immune regulation"
**作者**: Smith A, et al.
**摘要**: 研究STAT5B蛋白在肿瘤微环境中的双重作用，利用特异性抗体验证其在癌细胞增殖和免疫逃逸中的调控机制。
2. **文献名称**: "Development and validation of a phospho-specific STAT5B antibody for leukemia research"
**作者**: Zhang Y, et al.
**摘要**: 开发并验证了一种针对STAT5B磷酸化位点的抗体，应用于急性髓系白血病(AML)样本检测，揭示其异常激活与预后的相关性。
3. **文献名称**: "STAT5B interacts with glucocorticoid receptor in metabolic syndrome models"
**作者**: Lee H, et al.
**摘要**: 通过免疫共沉淀(Co-IP)结合STAT5B抗体，阐明STAT5B与糖皮质激素受体在代谢综合征中的相互作用及其对脂代谢的影响。
(注：以上文献为示例，实际引用需以真实数据库检索结果为准。)
The STAT5B antibody is a crucial tool in molecular and cellular biology research, targeting the Signal Transducer and Activator of Transcription 5B (STAT5B) protein. STAT5B, a member of the STAT family, plays a pivotal role in the JAK-STAT signaling pathway, mediating cellular responses to cytokines, growth factors, and hormones. Upon ligand-receptor binding, STAT5B is phosphorylated, dimerizes, and translocates to the nucleus to regulate gene expression involved in cell proliferation, differentiation, apoptosis, and immune regulation.
STAT5B is closely related to STAT5A, sharing 96% amino acid identity, but exhibits distinct roles in immune function, metabolism, and oncogenesis. Dysregulation of STAT5B is implicated in cancers (e.g., leukemias, breast cancer), autoimmune disorders, and metabolic diseases. The STAT5B antibody enables researchers to detect and quantify STAT5B expression, phosphorylation status, and subcellular localization using techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry. Specific antibodies distinguishing STAT5B from STAT5A are essential due to their overlapping functions.
Phospho-specific STAT5B antibodies (detecting tyrosine Y699 or serine S731 phosphorylation) are particularly valuable for studying pathway activation. These antibodies aid in understanding disease mechanisms, drug development, and biomarker discovery. Validation parameters such as species reactivity, application compatibility, and cross-reactivity with STAT5A should be carefully verified for experimental accuracy.
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