| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/500-1/2000 | Human,Mouse,Rat |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human COX10 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于COX10抗体的示例性参考文献(内容为模拟生成，建议通过学术数据库核实真实文献)：
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1. **文献名称**: *COX10 mutations in mitochondrial disorders: functional analysis using patient fibroblasts*
**作者**: Antonarakis, S. et al.
**摘要**: 本研究利用COX10抗体进行免疫印迹和免疫荧光，分析线粒体疾病患者成纤维细胞中COX10蛋白的表达及定位，发现特定突变导致COX10稳定性下降，影响细胞色素c氧化酶(COX)组装，证实其与 Leigh 综合征的关联。
2. **文献名称**: *Downregulation of COX10 in colorectal cancer correlates with tumor progression*
**作者**: Johnson, R. & Wang, L.
**摘要**: 通过免疫组化(COX10抗体)和qRT-PCR检测结直肠癌组织，发现COX10表达显著降低与肿瘤分期、转移及患者生存率缩短相关，提示其作为潜在预后标志物的价值。
3. **文献名称**: *COX10 antibody-based profiling of mitochondrial dysfunction in Parkinson’s disease models*
**作者**: Chen, H. et al.
**摘要**: 在帕金森病细胞模型中，使用COX10抗体结合流式细胞术发现线粒体COX10蛋白水平降低，导致复合体IV活性受损，抗氧化剂干预部分恢复其功能，为线粒体靶向治疗提供依据。
4. **文献名称**: *Tissue-specific expression of COX10 in murine development: insights from knockout models*
**作者**: Martinez, P. et al.
**摘要**: 通过COX10抗体检测基因敲除小鼠不同组织，揭示COX10在心脏和肌肉中的高表达及其发育依赖性，胚胎期COX10缺失引发严重线粒体缺陷，证实其对器官形成的必要性。
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建议通过 **PubMed** 或 **Web of Science** 搜索关键词 "COX10 antibody" + 研究领域(如癌症、线粒体疾病)获取真实文献。
The COX10 antibody is a crucial tool in studying the function and expression of the COX10 protein, which plays a pivotal role in mitochondrial energy production. COX10 (cytochrome c oxidase assembly factor 10) is a nuclear-encoded enzyme essential for the biosynthesis of cytochrome c oxidase (COX), the terminal enzyme in the mitochondrial electron transport chain (ETC). Specifically, COX10 catalyzes the conversion of protoheme to heme O, a critical step in the synthesis of heme A, a prosthetic group required for COX enzymatic activity. Dysregulation of COX10 has been linked to mitochondrial disorders, neurodegenerative diseases, and cancer, making it a biomarker of interest in metabolic and pathological studies.
Antibodies targeting COX10 enable researchers to detect and quantify its expression in tissues or cultured cells, often via techniques like Western blotting, immunohistochemistry, or immunofluorescence. These antibodies help investigate COX10's role in mitochondrial dysfunction, oxidative phosphorylation efficiency, and cellular responses to stress. Validated COX10 antibodies are particularly valuable in diagnosing mitochondrial diseases (e.g., Leigh syndrome) and exploring therapeutic strategies targeting mitochondrial metabolism. However, specificity and cross-reactivity challenges require careful validation using knockout controls or siRNA-based approaches. As COX10 levels often correlate with mitochondrial health, its antibody serves as a key reagent in studies linking energy metabolism to disease mechanisms, aging, and apoptosis.
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