| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/500-1/2000 | Human,Mouse,Rat |
| Aliases | hCOX16; HSPC203; C14orf112 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human COX16 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于COX16抗体的3篇参考文献示例(基于公开信息模拟，建议通过PubMed或Google Scholar验证具体内容)：
1. **标题**：*COX16 is required for assembly of mitochondrial complex IV and human cellular respiration*
**作者**：A. Ghezzi et al.
**摘要**：该研究利用COX16特异性抗体，通过Western blot和免疫荧光技术，证明COX16在人类线粒体复合物IV(COX)的组装中起关键作用，其缺失导致呼吸链功能障碍。
2. **标题**：*Characterization of COX16 function in yeast and mammalian mitochondria*
**作者**：M. Mick et al.
**摘要**：通过COX16抗体进行亚细胞定位分析，发现COX16与线粒体内膜相关，并揭示了其在酵母和哺乳动物中保守的COX组装调控机制。
3. **标题**：*A mutation in COX16 disrupts cytochrome c oxidase assembly in a patient with mitochondrial encephalomyopathy*
**作者**：S. Repp et al.
**摘要**：研究利用患者细胞系中的COX16抗体检测蛋白表达水平，发现COX16基因突变导致复合物IV组装缺陷，进而引发线粒体脑肌病。
**注意**：以上文献信息为模拟示例，实际文献需通过学术数据库检索确认。
COX16 (Cytochrome c oxidase assembly protein 16) is a nuclear-encoded mitochondrial protein critical for the biogenesis of the cytochrome c oxidase (COX) complex, the terminal enzyme in the mitochondrial electron transport chain. Discovered through studies of yeast homologs, human COX16 is localized to the mitochondrial inner membrane and facilitates the assembly of COX subunits, particularly stabilizing the maturation of the catalytic core subunit COX2. It interacts with other assembly factors, such as COX14 and COA3. to coordinate copper insertion into the COX1 and COX2 subunits, a vital step for enzymatic activity.
COX16 antibodies are essential tools for investigating mitochondrial respiratory chain disorders and metabolic diseases linked to COX dysfunction. These antibodies enable the detection and quantification of COX16 protein levels in tissues or cultured cells via techniques like Western blotting, immunofluorescence, and immunohistochemistry. Researchers use them to study COX16's role in cellular energy production, its tissue-specific expression patterns, and its involvement in pathologies such as neurodegenerative diseases, cardiomyopathies, and Leigh syndrome. Commercially available COX16 antibodies are typically raised in rabbits or mice using peptide antigens corresponding to conserved regions of the protein. Validation includes testing in knockout models or siRNA-treated cells to confirm specificity. Understanding COX16's function through antibody-based research provides insights into mitochondrial biology and potential therapeutic targets for oxidative phosphorylation deficiencies.
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