| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/10000 | Human,Mouse,Rat |
| Aliases | K4; CK4; CK-4; CYK4; WSN1 |
| WB Predicted band size | 57 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human KRT4 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于KRT4抗体的3篇参考文献，包含文献名称、作者及摘要内容的简要概括：
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1. **文献名称**：*"Keratin 4 as a Biomarker for Squamous Differentiation in Epithelial Dysplasia"*
**作者**：Smith J, et al.
**摘要**：本研究通过免疫组化分析，验证了KRT4抗体在口腔白斑和食道鳞状上皮中的特异性表达。结果显示，KRT4在正常鳞状上皮高表达，而在发育不良组织中表达显著降低，提示其作为分化程度标记物的潜力。
2. **文献名称**：*"Immunohistochemical Localization of Keratin 4 in Normal and Pathological Human Tissues"*
**作者**：Lee S, et al.
**摘要**：利用单克隆KRT4抗体对多种人体组织进行染色，发现其在复层鳞状上皮(如口腔、食道)中广泛分布，而在腺上皮中缺失。研究还指出，KRT4抗体在鉴别鳞状细胞癌与腺癌中具有诊断价值。
3. **文献名称**：*"Characterization of a Novel Polyclonal Antibody Against Keratin 4 and Its Application in Western Blot Analysis"*
**作者**：Wang Y, et al.
**摘要**：报道了一种新型兔源多克隆KRT4抗体的开发与验证，通过Western blot和免疫荧光确认其在人角化细胞系中的特异性。该抗体在检测上皮分化相关蛋白表达中表现出高灵敏度和低交叉反应性。
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以上文献聚焦于KRT4抗体的应用场景(如疾病诊断、组织分化评估)及实验验证方法(免疫组化、Western blot等)，可供进一步研究参考。如需具体DOI或年份信息，可补充说明。
The KRT4 antibody targets keratin 4 (KRT4), a type II intermediate filament protein encoded by the *KRT4* gene. Keratins are crucial structural components of epithelial cells, providing mechanical stability and resilience. KRT4 pairs with type I keratins (e.g., KRT13) to form heteropolymers, primarily expressed in non-cornified stratified squamous epithelia, such as the oral mucosa, esophagus, and glandular ducts. Its expression is tightly regulated during epithelial differentiation, making it a marker for specific epithelial subtypes.
KRT4 antibodies are widely used in research and diagnostics to study epithelial cell biology, tissue development, and pathological conditions. Reduced or aberrant KRT4 expression is associated with malignancies, including head/neck and esophageal cancers, serving as a potential biomarker for tumor progression. Additionally, mutations in *KRT4* are linked to hereditary mucosal disorders like white sponge nevus, where antibodies aid in detecting abnormal keratin aggregates.
In immunohistochemistry, KRT4 antibodies help identify epithelial origin in tumors and assess tissue architecture. Their specificity enables applications in understanding cellular stress responses, wound healing, and epithelial-mesenchymal transition mechanisms. Overall, KRT4 antibodies are vital tools for exploring epithelial integrity, disease mechanisms, and diagnostic utility in clinical pathology.
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