| WB | 咨询技术 | Bovine |
| IF | 咨询技术 | Bovine |
| IHC | 咨询技术 | Bovine |
| ICC | 技术咨询 | Bovine |
| FCM | 咨询技术 | Bovine |
| Elisa | 1/2000-1/5000 | Bovine |
| WB Predicted band size | 69 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Bovine |
| Immunogen | Bovine serum albumin, fraction V |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇与BSA抗体相关的文献示例(内容为模拟概括，非真实文献)：
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1. **文献名称**：*Production and Characterization of Polyclonal Antibodies Against Bovine Serum Albumin*
**作者**：Smith J, et al.
**摘要**：本文描述了通过免疫兔子制备抗BSA多克隆抗体的方法，分析了抗体的效价、特异性及交叉反应性，证实其在ELISA和免疫印迹中的应用效果。
2. **文献名称**：*BSA as a Carrier Protein in Hapten Immunoassays: Advantages and Limitations*
**作者**：Lee C, et al.
**摘要**：探讨BSA作为半抗原载体蛋白在抗体生成中的作用，比较其与其他载体蛋白(如KLH)的优缺点，并评估抗体对BSA-半抗原复合物的结合能力。
3. **文献名称**：*Reducing Non-Specific Binding in Immunoassays Using BSA Blocking Agents*
**作者**：Wang H, et al.
**摘要**：研究BSA作为封闭剂在免疫检测(如Western blot和ELISA)中的作用机制，优化封闭条件以降低非特异性信号，提高检测灵敏度。
4. **文献名称**：*Cross-Reactivity of Anti-BSA Antibodies with Human Serum Albumin: Implications for Diagnostic Assays*
**作者**：Garcia R, et al.
**摘要**：分析抗BSA抗体与人血清白蛋白(HSA)的交叉反应性，揭示其在临床诊断中可能产生的假阳性问题，并提出解决方案。
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注：以上文献信息为示例性质，实际引用时需根据具体研究查找真实发表的论文。
Bovine serum albumin (BSA) is a widely used protein in biochemical and immunological research, primarily known for its role as a blocking agent, stabilizer, or carrier molecule in assays like ELISA, Western blotting, and immunohistochemistry. Its small size (66 kDa), high solubility, and low cost make it ideal for reducing nonspecific binding by occupying unused sites on membranes or plates. However, BSA can inadvertently act as an immunogen when conjugated to haptens or antigens during antibody production. Antibodies against BSA (anti-BSA antibodies) often arise in polyclonal sera from animals immunized with BSA-linked antigens, leading to cross-reactivity in experiments. This can cause false-positive signals or high background noise if BSA is present in assay buffers or antigen preparations.
Additionally, BSA-specific antibodies may develop in humans or animals due to dietary exposure or prior immunological sensitization, complicating diagnostic tests or therapeutic applications. Researchers must account for anti-BSA reactivity by including appropriate controls (e.g., BSA-only lanes) or using alternative blocking agents like casein. Conversely, anti-BSA antibodies themselves serve as tools in quality control, verifying BSA removal in purification workflows or detecting residual BSA in biopharmaceuticals. Despite its ubiquity, BSA’s immunogenic potential underscores the need for careful experimental design to avoid artifacts.
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